000 02194nam a2200253 a 4500
001 vtls000045142
003 KUKTEM
005 20251114204422.0
008 100219t2009 my ao f m 000 0 eng d
020 _aTHE0004125(Local)
039 9 _a201905132110
_bfarhana
_c201108030925
_dFida
_c201107132307
_dVLOAD
_y201002191101
_zkam
040 _aUMP
090 _aTP156.F4 H57 2009 rs Bc.
100 0 _aMohd Hishafi Husin
245 1 0 _aScale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L /
_cMohd Hishafi Bin Husin
246 3 _aScale up of biopolymer (PHB) fermentation from shake flask to 10L stirred tank fermenter and optimization of levels of variables at 10L
_h[computer file]
260 _aKuantan :
_bUMP,
_c2009
300 _a62 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 computer disc
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2009
520 3 _aIn this research is about the fermentation process for PHB production by Cupriavidus necator using glucose as a substrate. The different parameters of glucose concentration and agitation speed were studied. There are two stages in this research. For the first stage, the scale-up process was done from shake flask to 10L fermenter based on constant volumetric mass transfer coefficient KLa. The shake flask agitation speed of 200 rpm and temperature 30°C were used as the basis of scale up. The result gives the PHB yield for shake flask and 10L as 60.12mg/L and 60.19 mg/L respectively. From these results, it can be concluded that the first stage had achieved its objective. For the second stage, optimization of variables was done by using glucose concentration; 10g/L and 30g/L and agitation speed; 200 rpm and 260 rpm as the manipulated variables. It was observed that the PHB yield is affected by initial glucose concentration of glucose used and also by the agitation speed.
650 0 _aBiopolymers
650 0 _aFermentation
999 _aVIRTUA40
_c1705
_d1711
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*6501*9992