| 000 | 02446nam a2200241 a 4500 | ||
|---|---|---|---|
| 001 | vtls000045140 | ||
| 003 | KUKTEM | ||
| 005 | 20251114204422.0 | ||
| 008 | 100219t2009 my ao f m 000 0 eng d | ||
| 020 | _aTHE0002290(Local) | ||
| 039 | 9 |
_a201905241223 _bshamsul _c201108030917 _dFida _c201107132307 _dVLOAD _y201002191023 _zkam |
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| 040 | _aUMP | ||
| 090 | _aQR186.85 .S53 2009 rs Bc. | ||
| 100 | 0 | _aMohd Shamsul Husin | |
| 245 | 1 | 0 |
_aSimulation study of monoclonal antibody production using superpro, upstream process / _cMohd Shamsul Bin Husin |
| 246 | 3 |
_aSimulation study of monoclonal antibody production using superpro, upstream process _h[computer file] |
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| 260 | _aKuantan : UMP, 2009 | ||
| 300 |
_a30 p. : _bill. (some col.) ; _c30 cm. + _e1 computer disc |
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| 502 | _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2009 | ||
| 520 | 3 | _aThe purpose of this study is to approximate hybridoma growth kinetic model by comparing simulation result from SuperPro Designer® and experimental result. Modeling of hybridoma include calculation of mass for 1 cell and its density. Two kinetic models tested in this study; experimental correlation and de Tremblay et al. (1992). Simplification need to be made as this will allow selected model to be used in SPD. Value of μmax, KsGLN, KsGLC are 1.09 d-1 (0.05 h-1), 0.3 mM (43.85 mg/L) and 0.1 mM (18.02 mg/L) respectively for de Tremblay and for experimental correlation, their values are 0.158 h-1, 0.0016 mM (0.23 mg/L) and 12.05 mM (2170.93 mg/L) respectively. Experimental data shows no stationary phase but simulation results show stationary phase. From simulation, cell count of 195907.7 while final concentration of glucose, glutamine, ammonia and lactate are 16.38, 4.07, 3.01 and 5.59 mmol/L respectively (experimental correlation) and cell count of 196185.73 while final concentration of glucose, glutamine, ammonia and lactate are 10.73, 2.09, 6.07 and 11.30 mmol/L respectively (de Tremblay). Serious deviations occurred because of simplification on de Tremblay model and inaccurate prediction of glutamine effect on hybridoma’s growth. Metabolic reaction used without taking hybridoma real behavior into account. Simulation shows exponential phase without having going through lag phase. | |
| 650 | 0 | _aMonoclonal antibodies | |
| 999 |
_aVIRTUA40 _c1708 _d1714 |
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| 999 | _aVTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*9992 | ||