000 02710nam a2200241 a 4500
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008 100219t2009 my ao f m 000 0 eng d
020 _aTHE0002256(Local)
039 9 _a201905241125
_bshamsul
_c201111241021
_dFida
_c201108030926
_dFida
_c201107132307
_dVLOAD
_y201002191125
_zkam
040 _aUMP
090 _aQP609.C39 N43 2009 rs Bc.
100 0 _aNor Fadzillah Hanis Abu Sulaiman
245 1 0 _aOptimization of inoculum size & agitation rate on expression of recombinant chitinase in Escherichia coli /
_cNor Fadzillah Hanis Binti Abu Sulaiman
260 _aKuantan :
_bUMP,
_c2009
300 _a45 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 computer disc
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2009
520 3 _aChitinase generally referred as endochitinases is randomly hydrolyzing chitin to produce N-acetyl-glucosamine. In addition, chitinase play an important role in chitin metabolism of microorganism for production of carbon and energy source. Chitinase can be found in bacteria, fungi, virus and higher plant. There are a lot of applications of chitinase either in pharmaceutical, biopestisides or food industry. For this research, chitinase was expressed in recombinant bacteria using Escherichia Coli as a host. The optimization was carried out by using response surface methodology. Focusing on two parameters which are inoculum size and agitation rate, the optimum condition was identified to enhance the process of chitinase production. The chitinase was expressed in different inoculum size at 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mL. Then the experiment continued observed in different agitation rate at 50, 100, 150, 200, 250 and 300 rpm. After overall observation, the best range of inoculum size gave between 0.8 mL to 1.2 mL and the range of agitation rate was identified between 230 rpm to 270 rpm. The best range of culture conditions was used for further study using Respond Surface Methodology (RSM). The optimal set of culture conditions for high soluble recombinant chitinase was determined as an inoculum size of 0.98 mL and 244.7 rpm of agitation rate. From the experimental value, the optimal production of recombinant chitinase was achieved 0.376 U/mL. This shows the 1.57-fold increment compared to the initial experiment which produced 0.239 U/mL of chitinase activity. These conditions successfully enhance the production of chitinase by using recombinant protein in Escherichia coli.
650 0 _aChitinase
650 0 _aEscherichia coli
999 _aVIRTUA40
_c1713
_d1719
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5200*6500*6501*9992