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008 100430t2010 my a f m 000 0 eng dd
020 _aTHE0002288(Local)
039 9 _a201905241221
_bshamsul
_c201108030915
_dFida
_c201107132316
_dVLOAD
_c201004301112
_dida
_y201004301056
_zida
040 _aUMP
090 _aQR186.85 .I43 2009 rs Bc.
100 0 _aMuhamad Nur Iman Ahmad Razali
245 1 2 _aA simulation study on downstream processing of monoclonal antibodies production using superpro designer /
_cMuhamad Nur Iman Bin Ahmad Razali
246 3 _aA simulation study on downstream processing of monoclonal antibodies production using superpro designer
_h[computer file]
260 _aKuantan, Pahang :
_bUMP,
_c2009
300 _axiv, 53 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 computer disc
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009
504 _aBibliography : p. 51-52
520 3 _aThe production of monoclonal antibodies and Fc fusion proteins form the largest and most rapidly expanding category of the biopharmaceuticals today with the annual sales exceeding USD$8 billion with the application across a wide range of diseases especially for the immunotherapy purpose. With the growth of this class of biomolecules, significant thought on the most efficient downstream processing steps for monoclonal antibodies has been taken into the highly consideration by the manufacturer in order to obtain the highest yield with the highest purity of the product which is why the simulation study are crucial in this development. The objectives of this study are to propose the most suitable framework of downstream processing for monoclonal antibodies production in order to achieve highest purities of mAbs and to stimulate the propose framework using SuperPro Designer® simulator. The process was initially done via the disk stack centrifuge and followed by microfiltration in order to harvest the protein. Three column of chromatography (Protein A affinity, anion exchange and hydrophobic interaction) were used to obtain the highest purity of the therapeutic. Viral inactivation also was employed to free the product from the endotoxins and pyrogens. The propose steps then, was introduced into the SuperPro Designer® simulator and the result obtained were 100% purity of IgG with 9.49345 g/L of concentration and 0.16687 kg/batch with 76.3% of recovery of the product.
650 0 _aMonoclonal antibodies
999 _aVIRTUA40
_c1759
_d1765
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5040*5200*6500*9992