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020 _aTHE0002186(Local)
039 9 _a201905131712
_byusri
_c201112051409
_dFida
_c201108030912
_dFida
_c201107132316
_dVLOAD
_y201004301516
_zida
040 _aUMP
090 _aQD702.C5 N67 2009 rs Bc.
100 0 _aNorheziwan Hamsah
245 1 0 _aOptimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology /
_cNorheziwan Bin Hamsah
260 _aKuantan, Pahang :
_bUMP,
_c2009
300 _axiv, 56 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 computer disc
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009
504 _aBibliography : p. [42]-49
520 3 _aThe function of chitinase as a chitin digestive enzyme has attracted the attention as one of the potential materials for control of phytopathogenic fungi and insect pests. However, chitinase is uneconomic for commercialized due to the high cost in its production. The development in recombinant DNA technology has helped to reduce chitinase production cost and enhance its production. The aim of this study is to determine the optimum conditions toward the production of recombinant chitinase in Escherichia coli by manipulating the concentration of inducers (IPTG and lactose). The best concentration of IPTG and lactose as inducers were determined by using the conventional method. Results from conventional method were used for further improvement of optimization by using Response Surface Methodology (RSM). In Response Surface Methodology, the optimization of expression was carried out based on the Central Composite Design (CCD). The effect of the inducers concentration toward the expression level of the recombinant chitinase production were analyzed and optimum values of tested variables for the production of chitinase were 1.19 mM IPTG and 0.98% lactose. Response surface analysis revealed that 0.513 U/ml of chitinase activity was achieved by using the optimized condition. This improved about 13.2-fold compare to initial experiment which produced 0.039 U/ml. This showed that combination between IPTG and lactose has increased the production of recombinant chitinase.
650 0 _aChitin
650 0 _aResponse surfaces (Statistics)
999 _aVIRTUA40
_c1765
_d1771
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992