000 02569nam a2200253 a 4500
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003 KUKTEM
005 20251114204433.0
008 120329t2010 my a f m 000 0 eng d
020 _aTHE0004534(Local)
039 9 _a201905131601
_bazhar
_y201203291019
_zFida
040 _aUMP
090 _aTP248.65.P76 R34 2010 rs Bc.
100 0 _aMohd.Raflie Maslih
245 1 0 _aSelective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique /
_cMohd.Raflie Maslih
260 _aKuantan :
_bUMP,
_c2010
300 _axiv, 53 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010
504 _aBibliography: p. 41-44
520 3 _aThe aim for this research is to produces protein separation tool by a new technique to separate protein molecule from protein solution. The technique used is the molecularly imprinted polymer, MIP. In this technique the target molecule that we want to separate will be the template and it mixed with selected monomers. Through polymerization process the template molecule will trapped between the polymer matrix that become solid and stable in term of heat and chemical. To make it has high selectivity on protein molecule the solid polymer is pounded to fine particles and through hydrolysis process the template is extracted and will leave complementary sequences that have exact configuration like the template shape. And further, two type of study were carried out, the first was analysis of particles structure in characterization of particles by using FT-IR spectrum and the second was study on the performances of this technique by conducting three experiments. The first experiment was the adsorption of BSA protein in MIP or synthesized polymer particles, the second experiment was the effect of protein concentration on separation and third experiment was to observe the effect of particles mass on separation. Polymerization and hydrolysis was successfully occur and it shown by the FT-IR spectrum. Experiment show that adsorption of protein was high for MIP particle that was 52.5 % while adsorption for NIP particle was 33.0 %. For the effect of concentration and mass of particle, it shows that the availability particle is the main factor that influence the amount of substrate separated
650 0 _aProteins
_xSeparation
650 0 _aSeparation (Technology)
999 _aVIRTUA40
_c2023
_d2029
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992