000 02401nam a2200253 a 4500
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003 KUKTEM
005 20251114204439.0
008 111201t2010 my a f m 000 0 eng d
020 _aTHE0004355(Local)
039 9 _a201905161046
_brohana
_y201112010842
_zFida
040 _aUMP
090 _aTP248.E5 R67 2010 rs Bc.
100 0 _aRoswani Shaimi
245 1 0 _aExtraction of papain enzyme from papaya leaves using enzyme assisted method /
_cRoswani Shaimi
260 _aKuantan, Pahang :
_bUMP,
_c2010
300 _axii, 61 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 computer disc
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) --- Universiti Malaysia Pahang - 2010
504 _aBibliography : p. 49-52
520 3 _aPapain is highly appreciated in medical field that prevents several chronic diseases such as cardiovascular disease, cancer and diabetes. Therefore, the objective of the research is to extract papain enzyme from papaya leaves using enzyme assisted method and determine papain activity. The Cellullase assisted extraction process was optimized by varying different parameters such as pH of extraction process, Cellulase concentration, Solid to Liquid ratio, incubation time and incubation temperature. Each 5 g of papaya leaves were ground and mixed with acetate buffer at a different Solid to Liquid ratio (1:5 - 1:25 g/ml) and adjusted with different pH (3-8). Cellulase was quantified and dispersed in acetate buffer (Na2HPO—citric acid) in different concentration (0.5 - 2.5 mg/ml).The enzymatic pretreatment was carried out and continued for enzyme assisted extraction process for various temperature and time (5070o4C and 1-5 h respectively). The optimal extraction conditions that satisfied the above constraints were found to be at pH 7, 1.5 mg/ml of Cellulase, solid to liquid of 1:10 (g/ml), an extraction time of 4 h and at an extraction temperature of 65oC. Under the optimum conditions, the extraction yield of papain was successfully achieved as much as 3.8018 µmole with 2.0910 Units/ml in Cellulase treated sample. Therefore, the potential for the papain enzyme in medical field can be fulfilled using enzyme assisted extraction.
650 0 _aEnzymes
_xBiotechnology
650 0 _aExtraction (Chemistry)
999 _aVIRTUA40
_c2181
_d2187
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992