| 000 | 01956nam a2200253 a 4500 | ||
|---|---|---|---|
| 001 | vtls000058553 | ||
| 003 | KUKTEM | ||
| 005 | 20251114204449.0 | ||
| 008 | 120305t2010 my da f m 000 0 eng d | ||
| 020 | _aTHE0004133(Local) | ||
| 039 | 9 |
_a201905132119 _bfarhana _c201203051313 _dida _y201203051106 _zFida |
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| 040 | _aUMP | ||
| 090 | _aTP156.F4 N67 2010 rs Bc. | ||
| 100 | 0 | _aNik Nor Aziati Abd Aziz | |
| 245 | 1 | 0 |
_aEffect of substrate concentration and ph on succinic acid production / _cNik Nor Aziati Abd Aziz |
| 260 |
_aKuantan, Pahang : _bUMP, _c2010 |
||
| 300 |
_axv, 95 p. : _bill. (some col.) ; _c30 cm. + _e1 CD-ROM |
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| 502 | _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 | ||
| 504 | _aBibliography: p. 60-62 | ||
| 520 | _aThe purpose of this research is to study the effect of substrate concentration and pH on succinic acid production. Succinic acid has been produced from the fermentation process by using Escherichia coli b-strain. The experiments were conducted in Modified Schott Bottle in incubator shaker to maintain anaerobic condition, temperature and agitation speed. Fermentation were carried out at the optimum temperature of E.coli growth which is 37 OC for 72 hours. The results of HPLC analysis showed that increase in fermentation time will increase succinic acid production. The maximum production of succinic acid is at pH 6.0. Initial substrate concentration (5 to 15 g/L) will increase Succinic acid production, but after 15 g/L substrate concentration, the succinic acid production will decrease. From this study, it was observed that the substrate concentration and pH are important factor that affects both growth and growth-associated productions of molecules to increased succinic acid production. | ||
| 650 | 0 | _aFermentation | |
| 650 | 0 | _aSuccinic acid | |
| 999 |
_aVIRTUA40 _c2486 _d2492 |
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| 999 | _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992 | ||