000			02791nam a2200241 a 4500
001			vtls000058181
003			KUKTEM
005			20251114204510.0
008			120110t2010 my da f m 000 0 eng d
020			_aTHE0004143(Local)
039		9	_a201905132128 _bfarhana _c201705231159 _dida _y201201101030 _zFida
040			_aUMP
090			_aTP156.F5 P66 2010 rs Bc.
100	0		_aPonnarasy A/P Ganasen
245	1	0	_aIsolation of albumin protein from goat’s milk using anion exchange chromatography / _cPoarasy A/P Gaase
260			_aKuantan, Pahang : _bUMP, _c2010
300			_avii, 79 p. : _bill. (some col.) ; _c30 cm. + _e1 CD-ROM
502			_aProject paper (Bachelor of Chemical Engineering (Biotechnology) -- Universiti Malaysia Pahang - 2010
504			_aBibliography: p. 76-79
520	3		_aMilk proteins have been extensively studied for over 90 years. The aim of this study was to introduce a simple, reproducible, and less expensive method which is ion exchange chromatography for isolation of albumin protein from goat’s milk. The main whey proteins are β-lactoglobuline and α- lactalbumin for which genetic variants are also known. The fraction of whey also contains substantial amounts of immunoglobulin and serum albumin. Whey (lactoserum) was obtained by isolating casein from defatted milk using hydrochloric acid. The proteins in the supernatant were fractionated using Q-Sepharose column chromatography for separation. This research were conducted to optimize the pH of the milk sample for separation process. The processes of the separation initialized with sample preparation followed by packing process of ion exchange column , buffer preparation, ion exchange running process and ending by analyzing the ion exchange fraction using reverse process chromatography (PRC). The pH that have been analyzed during this study were milk with pH 5.5, 6.5, 7.5 and 8.5. Result shows that ph 8.5 able to collect α-lactalbumin whereas in other pH does not so. Anion exchange chromatography able to isolate BSA and higher concentration was obtained at pH 5.5 with the amount, 0.03616164 mg/ml. RPC method is presented for the determination of albumin method is rapid, reliable and sensitive and gives good accuracy and precision for measurement of the whey proteins. The procedure developed is particularly suited to laboratory-scale applications, but it can also be applied to the purification on a large scale. Separation using simple process and local dairy product which is goat milk able to reduce the cost and time and have potential for commercialization. Standard curve used to analyze the data obtained from RPC analysis.
650		0	_aUltrafiltration
999			_aVIRTUA40 _c3078 _d3084
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992