000 02360nam a2200241 a 4500
001 vtls000058594
003 KUKTEM
005 20251114204511.0
008 120306t2010 my a f m 001 0 eng d
020 _aTHE0004536(Local)
039 9 _a201905311531
_bnazirah
_c201905131604
_dazhar
_y201203061335
_zida
040 _aUMP
090 _aTP248.65.P76 S86 2010 rs Bc.
100 0 _aSu Najwa Mohamed
245 1 0 _aProtein purification by using immobilied metal ions affinity zeolite adsorbent /
_cSu Najwa Mohamed
260 _aKuantan, Pahang :
_bUMP,
_c2010
300 _axv, 76 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2010
504 _aBibliography : p. [59]-62
520 3 _aThe applications for purified proteins have grown considerably over recent years due to their biological and nutritional properties. The aim of this research are to develop zeolite as inorganic stationary substrate in immobilized metal ion affinity (IMA) for protein purification and to study the optimum condition to purify protein in order to maximize yield and purity of protein by applying the principle of adsorption process. The effect of contact time, different types of adsorbent and various pH values were studied in a batch experiment by using Bovine Serum Albumin (BSA) as protein sample. The sorption equilibrium was reached within 10 minutes. Fe3+ ion showed the best performance for BSA and has the suitable interaction with both HY and HBeta zeolite framework and the protein as compared to Pb2+, Ni2+ ions. The adsorption capacity for BSA was found to be the highest at pH 4.5 that is close to isoelectric point (pI) with Fe- HBeta used as adsorbent. The adsorbed amount increases as the pH increases and reduces as the pH exceeds the pI value. This result proves that adsorption capacity of Bovine Serum Albumin depends on the types of adsorbent used, which means the adsorption stoichiometries depends on physical characteristic of adsorbent that could increase and reduce the adsorption capacity of the protein as well as the pH of protein. The adsorption isotherm data of BSA is well fitted to the Langmuir isotherm model.
650 0 _aProtein
_xPurification
999 _aVIRTUA40
_c3110
_d3116
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992