000			01949nam a2200253 a 4500
001			vtls000058544
003			KUKTEM
005			20251114204512.0
008			120302t2010 my a f m 000 0 eng d
020			_aTHE0004348(Local)
039		9	_a201905161036 _brohana _c201203021211 _dFida _y201203021210 _zFida
040			_aUMP
090			_aTP248.E5 D53 2010 rs Bc.
100	0		_aNur Dianaty Nordin
245	1	4	_aThe extraction of papain from papaya leaves / _cNur Dianaty Nordin
260			_aKuantan, Pahang : _bUMP, _c2010
300			_axv, 45 p. : _bill. (some col.) ; _c30 cm. + _e1 CD-ROM
502			_aProject paper (Bachelor of Chemical Engineering (Biotechnology)) --- Universiti Malaysia Pahang - 2010
504			_aBibliography : p. 38-41
520	3		_aThe use of papaya leaves to extract papain enzyme was evaluated for different extraction technique mainly using grinding method, enzyme-assisted extraction and ultrasonication. Enzyme concentration was then determined by measuring the amount of amino acid released in the reaction of protease assay. The combination of grinding method, enzyme-assisted extraction was proven to give highest yield of papain by 36.75% when compared to grinding alone. Then further purification of the extracted papain was done by using ammonium sulfate precipitation. The optimized concentration of ammonium sulfate saturation was achieved at 55% concentration in order to fully precipitate the protein. The combination of grinding, enzyme-assisted extraction and ultrasonication can be considered as a potential extraction technique to extract papain as it is more delicate towards protein extraction. Furthermore, the investigation on other source of papain such as from the petioles, stalks and stem could also be done.
650		0	_aEnzymes _xBiotechnology
650		0	_aExtraction (Chemistry)
999			_aVIRTUA40 _c3119 _d3125
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992