000 02599nam a2200241 a 4500
001 vtls000018739
003 KUKTEM
005 20251114204512.0
008 070315t2006 my f m 000 0 eng d
020 _aTHE0004495(Local)
039 9 _a201905170904
_brohana
_c201204161535
_dida
_c201204161534
_dida
_c201204161534
_dida
_y200703151507
_zkam
040 _aUMP
090 _aTP248.65.C44 N39 2006 rs Thesis
100 0 _aMohd. Nazip Kassebullah
245 1 0 _aProduction of cellulase enzyme from Aspergillus terreues SUK-1 using sugar cane waste :
_bthe effect of substrate concentration and assay temperature /
_cMohd Nazip Bin Kassebullah
260 _aKuantan, Pahang :
_bKUKTEM,
_c2006
300 _a54 p. :
_bill. ;
_c30 cm. +
_e1 compter disc
502 _aProject paper (Bachelor of Chemical Engineering) -- Kolej Universiti Kejuruteraan & Teknologi Malaysia - 2006
520 _aNatural by product of agricultural waste can be turned to products of commercial interests such as ethanol, glucose and single cell protein. Much effort from scientists and researches all around the world has been put to extend the full use of agricultural waste. Reports of cellulase enzyme production from the bioconversion of lignocellulosics materials has much been made. However there is still much space to find the most suitable condition. In this research, sugar cane bagasse which is used as substrate is subjected to physical treatment using milling and grinding. After that, sugar cane bagasse is use as substrate in the production of cellulase enzyme using Aspergillus terreus SUK-1 fungus. Cellulase enzyme production was studied using submerged fermentation under shaking. The production was run in a lab scale using Erlenmeyer flask only. After that the enzyme was assayed at different temperature. The purpose of this research was to investigate the effect of substrate concentration on yield of cellulase and the optimum temperature where the yield of enzyme was the highest. Analysis is based on the activity of carboxymethylcellulase and xylanase enzymes. Result shows that the peak of xylanase activities was 0.011322 U/ml on day 5 using 5g SCB as substrate and fermentation temperature of 50°C while carboxymethylcellulase peak was 0.007985049 U/ml of activity using 3g SCB as substrate and fermentation temperature of 40°C. Xylanase activity was high when assayed at 50°C while carboxymethylcellulase activity was high when assayed at 40°C.
650 0 _aCellulase
650 0 _aAspergillus
999 _aVIRTUA40
_c3125
_d3131
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5200*6500*6501*9992