000			02910nam a2200265 a 4500
001			vtls000058708
003			KUKTEM
005			20251114204514.0
008			120319t2010 my a f m 000 0 eng d
020			_aTHE0004474(Local)
039		9	_a201905161618 _brohana _y201203190914 _zFida
040			_aUMP
090			_aTP248.27.M53 B35 2010 rs Bc.
100	0		_aBaiyinah A.Talip
245	1	0	_aEffect of inoculum concentration on butanol and ethanol production by clostridium acetobutylicum / _cBaiyinah A.Talip
260			_aKuantan, Pahang : _bUMP, _c2010
300			_axv, 84 p. : _bill. ; _c30 cm. + _e1 CD-ROM
502			_aProject paper (Bachelor of Chemical Engineering (Biotechnology) -- Universiti Malaysia Pahang - 2010
504			_aBibliography : p. [60]-64
520	3		_aIn Malaysia, the government has implement policy instruments aimed at promoting the production of butanol and ethanol as an alternative to renewable energy. The objective was to study the effect of inoculum concentration on butanol and ethanol production and besides that, the study also focused on growth profile of Clostridium acetobutylicum and glucose consumption in POME and RCM. The methods begin with palm oil mill effluent (POME) analysis using High Performance Liquid Chromatography (HPLC). The process continued with the microbial fermentation and each inoculums concentration at 5% v/v, 10 % v/v and 15 % v/v were prepared in Reinforced Clostridia Media (RCM). POME as an alternative medium was sedimented for 24 hours, and has been prepared at pH 5.8, diluted with 90 % substrate, deoxygenated the POME using nitrogen gas. Both medium POME and RCM were seeded with different inoculums concentration and after 3 days fermentation, the fermentation broth were undergo liquid-liquid extraction and prepared for Gas Chromatography (GC) analysis. The result obtained from POME analysis showed that galactose was the main component in POME. Moreover, the maximum butanol production for both POME and RCM medium were 0.18533% at 15% v/v (POME) and 0.00801% at 5% v/v (RCM). Result showed that increasing the inoculum concentration would reduce the fermentation time, increased the total glucose consumption and increased the butanol production. While the maximum production of ethanol were, 1.87593% at 15% v/v during 40 hours (POME) and 2.36754% at 5% v/v during 60 hours (RCM) of fermentation time. The result showed that there were significant correlation between inoculum concentration and butanol and ethanol production. Thus, it could be concluded that inoculum concentration does affect the butanol and ethanol production, besides, POME could be an alternative medium for butanol and ethanol fermentation.
650		0	_aMicrobial biotechnology
650		0	_aFermentation
650		0	_aButanol
999			_aVIRTUA40 _c3203 _d3209
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*6502*9992