| 000 | 02388nam a2200265 a 4500 | ||
|---|---|---|---|
| 001 | vtls000058627 | ||
| 003 | KUKTEM | ||
| 005 | 20251114204517.0 | ||
| 008 | 120312t2010 my a f m 000 0 eng d | ||
| 020 | _aTHE0004118(Local) | ||
| 039 | 9 |
_a201905131414 _bfarhana _y201203121032 _zida |
|
| 040 | _aUMP | ||
| 090 | _aTP156.F4 F37 2010 rs Bc. | ||
| 100 | 0 | _aFarizah Hamid | |
| 245 | 1 | 0 |
_aProduction of polyhyroxybutyrate (PHB) from Bacillus cereus by using sawdust as a substrate / _cFarizah Hamid |
| 260 |
_aKuantan, Pahang : _bUMP, _c2010 |
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| 300 |
_axii, 42 p. : _bill. (some col.) ; _c30 cm. + _e1 CD-ROM |
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| 502 | _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 | ||
| 504 | _aBibliography : p. 35-36 | ||
| 520 | 3 | _aThe objectives of this research are to determine the production of lignin degradation in sawdust using Bacillus cereus and to study the potential of Bacillus cereus for PHB production using sawdust as a substrate. There consist of two major parts which are fermentation for delignification of sawdust and production of PHB. For delignification analysis, Klason method is used in order to determine the percentage of lignin content and lignin degrades by using sawdust as a substrate. Bacillus cereus act to degrade lignin in the sample. Based on the experiment sample 1 shows the lower percent of lignin content with 38.45% and become the higher percentage of lignin degrade with 13.75%. This is because the bacteria which are Bacillus cereus break down the long carbohydrate chains or hemicellulose converted the sawdust into short chains of glucose and eventually individual glucose. While sample 4 shows the lowest percentage of lignin degrade but higher percentage of lignin content. The second part is on the production of PHB. From the result, percentage yield of PHB produced from cellulose and glucose are 1.29% and 3.42%. In conclusion, Bacillus cereus has potential to produce PHB using sawdust as a substrate although the yield is smaller. Several precautions must take placed to avoid any mistaken during the experiment. As a recommendation HPLC can be used to obtain more accurate result. | |
| 650 | 0 | _aFermentation | |
| 650 | 0 | _aBiopolymers | |
| 650 | 0 |
_aLignin _xOxidation |
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| 999 |
_aVIRTUA40 _c3271 _d3277 |
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| 999 | _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*6502*9992 | ||