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005 20251114204517.0
008 120327t2010 my a f m 000 0 eng d
020 _aTHE0004533(Local)
039 9 _a201905131550
_bazhar
_c201203270925
_dFida
_y201203270925
_zFida
040 _aUMP
090 _aTP248.65.P76 M39 2010 rs Bc.
100 0 _aMohd Mazlan Mohamed
245 1 0 _aIsolation Of ß-Lactoglobulin and a-Lactalbumin from whey /
_cMohd Mazlan Mohamed
260 _aKuantan, Pahang :
_bUMP,
_c2010
300 _axii, 39 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010
504 _aBibliography : p. [30]-32
520 3 _aWhey is a byproduct in cheese production. Whey solution is rich with proteins; the main proteins in whey are β-Lactoglobulin (β-Lg) (50%), α-Lactalbumin (α-La) (20%), bovine serum albumin (BSA) (10%) and immunoglobulin’s (Igs) (10%). Single protein has higher value then whey solution and has specific application. Hence this paper aims to isolate two of main whey proteins that are β-Lg and α-La due to their functions and higher value of pure protein. In this work, whey was prepared during casein coagulation from skim milk by acidic precipitation at pH below than 5 with 0.5 M hydrochloric acid. The experiments were conducted by AKTA Fast Protein Liquid Chromatography (FPLC), using size exclusion (packed by superdex TM 75 gel filtration media in an empty size exclusion XK column (60 ml)) and cation exchange column (HiTrap SP HP, 1ml column) in order to isolate both proteins from whey and 10% separating gel of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) was used to identify each protein form isolation steps and to determine the degree of purity of β-Lg and α-La. Silver staining was used in developing protein band on SDS gel. The experimental results concluded that both β-Lg and α-La can be isolated from other proteins in whey by using size exclusion and cation exchange fast protein liquid chromatography. Both β-Lg and α-La were visualized at 18 kDa and 14 kDa respectively, in SDS-PAGE.
650 0 _aProteins
_xSeparation
650 0 _aSeparation (Technology)
999 _aVIRTUA40
_c3277
_d3283
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992