000			02149nam a2200241 a 4500
001			vtls000058590
003			KUKTEM
005			20251114204518.0
008			120306t2010 my a f m 000 0 eng d
020			_aTHE0004761(Local)
039		9	_a201905151057 _bsmfh _c201203061220 _dFida _y201203061219 _zFida
040			_aUMP
090			_aTP414 .S65 2010 rs Bc.
100	0		_aSiti Solehah Ahmad
245	1	0	_aEffect of temperature and pH on glucose production using enzymatic hydrolysis / _cSiti Solehah Ahmad
260			_aKuantan : _bUMP, _c2010
300			_axv, 89 p. : _bill. ; _c30 cm. + _e1 CD-ROM
502			_aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010
504			_aBibliography: p. 66-68
520	3		_aEnzymatic hydrolysis is one of the effective methods in glucose production due to enzyme properties which are highly specific and very sensitive. Therefore, this paper was designed to study the effect of temperature and pH on glucose production using enzymatic hydrolysis. By using enzymatic hydrolysis, cellulase from Trichoderma reesei was used and supplemented with cellobiase from Aspergillus niger for increasing the glucose production. Cellulose as a substrate was hydrolyzed by these enzymes to produce glucose and the amount of glucose production was determined by high-performance liquid chromatography (HPLC). The result showed that the highest glucose concentration was produced at the temperature of 50°C and pH 5.0 while the lowest glucose production was produced at 30°C and pH 6.5 which were 21.09 mg/ml and 3.55 mg/ml, respectively. By changes the temperature and pH above or below 50°C and pH 5.0 results in low glucose production because the enzyme was lose their three-dimensional functional shape due to disruption from unstable condition of the environment system. As a conclusion, all the requirement for complementary in the configuration of cellulose and enzyme must be considered seriously for the highest glucose production.
650		0	_aGlucose
999			_aVIRTUA40 _c3300 _d3306
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992