000 02565nam a2200241 a 4500
001 vtls000066748
003 KUKTEM
005 20251114204521.0
008 121121t2012 my a f m 000 0 eng d
020 _aTHE0004012(Local)
039 9 _a201905151225
_bfauzi
_y201211211409
_zida
040 _aUMP
090 _aTP156.E8 H39 2012 rs Bc.
100 0 _aHazwani Zulkiply
245 1 0 _aOptimization of extraction parameters of total phenolic compound from Cosmos caudatus /
_cHazwani Zulkiply
260 _aKuantan, Pahang :
_bUMP,
_c2012
300 _axiv, 46 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2012
504 _aBibliography : p. 29-32
520 3 _aThe wide ranges of extraction parameters used being identified from previous studies derives the need to find the best conditions to yield optimum extraction of total phenolic compounds from Cosmos caudatus. The objective of this research is to determine the optimum extraction parameters, namely ultrasonic frequency (from 30 to 70 kHz), sample-to-solvent ratio (from 2 to 10 w/v %) and extraction time (from 30 to 300 minutes) of total phenolic compound from Cosmos caudatus. The experimental design was first generated from Response Surface Methodology by using Design Expert 7.1.6 with three independent variables, namely ultrasonic frequency, sample-to-solvent ratio (SSR) and extraction time. Results showed that the optimization of extracting total phenolic compounds (TPC) from Cosmos caudatus can be accomplished by employing ultrasonic frequency of 70 kHz, 2g dry sample/100mL ethanol and extraction time of 300 minutes with yield of 7.7395 mg GAE/g dw which is in close agreement with the predicted value (7.5359 mg GAE/g dw). Analysis of variance showed significant ultrasonic frequency and sample-to-solvent ratio, but insignificant extraction time. This might be partly due to phenolic oxidation during the extraction itself. Since previous and present studies suggests that the extraction of total phenolic compounds can be further optimized, upcoming studies need to be directed at varying the significant extraction parameters including the extraction temperature, types of solvent used and extraction methods.
650 0 _aExtraction (Chemistry)
999 _aVIRTUA40
_c3384
_d3390
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992