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008 121227t2012 my a f m 000 0 eng d
020 _aTHE0004443(Local)
039 9 _a201905161539
_brohana
_y201212270929
_zFida
040 _aUMP
090 _aTP248.25.N35 A28 2012 rs Bc.
100 0 _aAbu Hassan Mohd Nazir
245 1 0 _aOptimization of bacterial cellulose production in apple juice medium by using response surface methodology (RSM) /
_cAbu Hassan Mohd Nazir
260 _aKuantan, Pahang :
_bUMP,
_c2012
300 _axiv, 48 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology) -- Universiti Malaysia Pahang - 2012
504 _aBibliography : p. 43-46
520 3 _aAcetobacter xylinum is a type of acetic acid producing bacteria that can synthesis bacterial cellulose from carbohydrates. Bacterial cellulose that produced has high purity, high water holding capacity, good mechanical strength, elasticity, high crystallinity and high porosity compare to plant cellulose. This research was using apple juice as the high potential carbon sources to replace the pure carbon sources as the substrate for the synthesis of bacterial cellulose. The objective of this study was to optimize bacterial cellulose production in apple juice medium by using Response Surface Methodology (RSM). The research will be conducted by using 5 samples with difference temperature (28ºC, 29ºC, 30ºC, 31ºC and 32ºC) , 5 sample with difference in pH (4, 5, 6 ,7 and 8) and 5 sample with different medium concentration (60 %, 70%, 80%,90% and 100%). Each sample contains 100mL of medium in 250mL conical flask and incubated in incubator for 5 days. The bacterial cellulose film produced by Acetobacter Xylinum was treated with 1% Natrium Hydroxide (NaOH) for 1 day and then washed with Deionized water to neutralize the bacterial cellulose. The result showed that the medium concentration, pH and temperature of cultivation were affected the production yield of bacterial cellulose. By using response surface methodology (RSM), the optimum condition for bacterial cellulose production was 95% (v/v) for medium concentration, pH 5.95 and cultivation temperature at 30.3ºC. The film then was analyzed by using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). By using FTIR the hydrogen bonds (-OH) of bacterial cellulose was determined while by using SEM the interwoven strands, ribbon-like (microfibrils) of bacterial cellulose structure was observed. Thus, the optimum conditions for bacterial cellulose production can be determined by using RSM.
650 0 _aPolymeric composites
650 0 _aBiopolymer
999 _aVIRTUA40
_c3399
_d3405
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992