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001			vtls000068066
003			KUKTEM
005			20251114204522.0
008			130110t2012 my a f m 000 0 eng|d
020			_aTHE0003878(Local)
039		9	_a201905161515 _bshah _y201301101045 _zFida
040			_aUMP
090			_aTP156.C5 A37 2012 rs Bc.
100	0		_aNur Aishah Amalin Ramjis
245	1	0	_aPreparation of cation-exchange membrane chromatography through modification of polyamide membrane by grafting of acrylic acid monomer / _cNur Aishah Amalin Ramjis
260			_aKuantan, Pahang : _bUMP, _c2012
300			_axiv, 59 p. : _bill. ; _c30 cm. + _e1 CD-ROM
502			_aProject paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2012
504			_aBibliography : p. 35-37
520	3		_aPacked bed chromatography is widely used for protein separation and purification. However some limitation of packed bed chromatography has been identified such as high-pressure drop, internal diffusion limitation and compaction for the soft beads. This encourages people to use membrane chromatography as alternative to the packed bed chromatography. In this study, membrane chromatography was developed from polyamide-based membrane by grafting acrylic acid (AA) monomer to produce cation-exchanger membrane chromatography. During grafting process, the concentration of AA monomer from 0.1 M to 1.0 M and grafting time from 15 min to 120 min was studied. The optimum monomer concentration was achieved at 0.5 M with a binding capacity of 443.39 mg BSA/g membrane. The optimum reaction time is 45 minutes which gives a binding capacity of 533.40 BSA mg/g membranes. The BSA adsorption capacity can be related to the amount of carboxyl group present in the modified membrane surface after grafting process. The successful of membrane chromatography preparation depend on the careful optimization the chemical reaction parameters involve.
650		0	_aChromatography
650		0	_aChromatography _xAnalysis
999			_aVIRTUA40 _c3417 _d3423
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992