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020 _aTHE0003879(Local)
039 9 _a201905161515
_bshah
_c201302041534
_dhuda
_c201302041532
_dhuda
_c201301091648
_dFida
_y201301091558
_zFida
040 _aUMP
090 _aTP156.C5 A45 2012 rs Bc.
100 0 _aNurul Alia Khalil
245 1 0 _aPreparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer /
_cNurul Alia Khalil
260 _aKuantan, Pahang :
_bUMP,
_c2012
300 _axiii, 43 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2012
504 _aBibliography : p. 33-35
520 3 _aNowadays, with the increase demand of protein production and purity, the purification cost of the protein also increases about 50 to 90%. Packed bed chromatography is widely used in protein separation. However, there are some limitations of the packed bed chromatography such as high-pressure drop, channeling problem and complicated scale up process. Most of these limitations can be overcome by membrane chromatography. The main objective of this research is to prepare cation-exchange membrane chromatography from polyamide membrane by chemical grafting of methacrylic acid monomer. Potassium persulfate and potassium metabisulfite was used to generate the radicals in the polyamide membrane and grafted with methacrylic acid monomer together with ethylene glycol dimethacrylate as a cross-linker. Different monomer concentration between 0.1 and 1.0M and monomer grafting duration from 15 – 120 min was studied toward producing high protein binding capacity membrane. Optimum time for grafting methacrylic acid onto polyamide membrane was 45 minutes with the binding capacity 599.6390 mg/g BSA at the monomer concentration of 0.1 M. Identification and optimization of critical parameter in grafting process is crucial for the development of high performance membrane chromatography materials.
650 0 _aChromatography
650 0 _aChromatography
_xAnalysis
999 _aVIRTUA40
_c3450
_d3456
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992