000			02117nam a2200253 a 4500
001			vtls000067228
003			KUKTEM
005			20251114204533.0
008			121130t2012 my a f m 000 0 eng|d
020			_aTHE0003881(Local)
039		9	_a201905161514 _bshah _c201302041531 _dhuda _y201211301601 _zFida
040			_aUMP
090			_aTP156.C5 F37 2012 rs Bc.
100	0		_aNur Farahain Khusnun
245	1	0	_aMixed-mode interaction membrane chromatography for protein fractionation / _cNur Farahain Khusnun
260			_aKuantan, Pahang : _bUMP, _c2012
300			_axiv, 39 p. : _bill. ; _c30 cm. + _e1 CD-ROM
502			_aProject paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2012
504			_aBibliography : p. 31-32
520	3		_aNowadays, membrane chromatography has widely use in protein separation. Most of the membrane chromatography is prepared through a chemical modification which sometimes can changes the structure and properties of the membrane itself. In this study, membrane chromatography was prepared through physical modification using the mixed matrix membrane preparation concept. Amberlite IR120 cation exchange resin (CEX) and Lewatit MP500 anion exchange resin (AEX) was used to formulate mixed mode interaction membrane chromatography (MMIMC) based on ethylene vinyl alcohol base membrane matrix. The total resin loading from 20-50% and the ratio between anion exchange resin and cation exchange resin was studied. This research found the optimum resin loading is 30%. Pure AEX MMM has the binding capacity of 340.26 mg BSA/g membrane and pure CEX MMM has a binding capacity of 3166.22 mg LZY/g membrane. The MMIMC with ratio CEX/AEX equal to 50/50 has a capacity of 895.898 mg LZY/g membrane and 3430.74 mg BSA/g membrane. A high performance MMIMC can be produced by optimization the loading percentage of ion exchanger resin and the ratio of CEX/AEX resin incorporate into the membrane.
650		0	_aChromatography
650		0	_aChromatography _xAnalysis
999			_aVIRTUA40 _c3749 _d3755
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992