000			02121nam a2200277 a 4500
001			vtls000076100
003			KUKTEM
005			20251114204539.0
008			131114t2013 my da f m 000 0 eng d
020			_aTHE0004969(Local)
039		9	_a201905131624 _bSHAHRILJ _y201311141650 _znabilah
040			_aUMP
090			_aTP1180.P6 N34 2013 rs Bc.
100	0		_aNahdiyyah Rosslan
245	1	0	_aInoculum development in 2L stirred tank fermenter for production of PHB biopolymer in a 20 L stirred tank fermentation on a medium of oil palm frond juice (OPFJ) / _cNahdiyyah Rosslan
260			_aKuantan, Pahang : _bUMP, _c2013
300			_axiv, 40 p. : _bill. ; _c30 cm. + _e1 CD-ROM
502			_aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
504			_aBibliography : p. 34-35
520	3		_aPolyhydroxybutyrate (PHB) is a biopolymer plastic as intracellular product of bacteria which can be degradable naturally. The purpose of this experiment is to scale up the production of PHB biopolymer by Cupriavidus Necator using oil palm frond juice (OPFJ) as carbon source. The process will involve fermentation process, the analysis on cell dry weight and concentration of PHB. The fermentation will be done in the 2 L stirred tank fermenter model Sartorius with the combination of 450 ml oil palm frond juice, 150 ml inoculum development 2, and 900 ml mineral salt medium (MSM) by manipulating the agitation speed and fixed the aeration rate at 1.5 L/min. Next, the cell dry weight and PHB will be analyse. From the result of DOT curve, 200 rpm in fermenter shows the same pattern as in 220 rpm in flask. 200 rpm is used in 2L fermentation. Highest PHB yield for fermentation in 2 L was 2.0650 g/L and highest cell dry weight was 5.7 g/L. While for cascade fermentation highest cell dry weight was 9.5 g/L and highest PHB content was 5.42 g/L.
650		0	_aPolyesters
650		0	_aBiopolymers
650		0	_aFermentation
650		0	_aOil palm
999			_aVIRTUA40 _c3910 _d3916
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*6502*6503*9992