000 02967nam a2200265 a 4500
001 vtls000075742
003 KUKTEM
005 20251114204543.0
008 131028t2013 my da f abm 000 0 eng d
020 _aTHE0004001(Local)
039 9 _a201905151035
_bfauzi
_c201310280837
_dnabilah
_y201310280834
_znabilah
040 _aUMP
090 _aTP156.E8 F37 2013 rs Bc.
100 0 _aNurfariha Fathmi Zakaria
245 1 0 _aApplication of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol /
_cby Nurfariha Fathmi Zakaria
260 _aKuantan, Pahang :
_bUMP,
_c2013
300 _axii, 38 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2013
504 _aBibliography : p. 31-34
520 3 _aThe mixture of s.rebaudiana stems and leaves will be extracted to get the stevioside. Stevioside is a diterpene steviol Glycoside which is produced by conventional process. It is used to sweeten soft drink,soya sauce, yoghurt and other food. It also good for diabetic patients. This research objective is to extract the steviol glycosides from the mixture of s.rebaudiana stems and leaves using ultrasound extraction technique and find their applications. The process normally include the extraction, pre-treatment, separation, purification and refining. These processes will use ultrasound technique and followed by High performance liquid chromatography analysis (HPLC). Ultrasound extraction technique is one of the techniques to extract the mixture of s.rebaudiana stems and leaves before the sample of the the mixture of s.rebaudiana stems and leaves is analyzed by HPLC. The mixture of s.rebaudiana stems and leaves will be in dry and powder condition before it extracted by ultrasound extraction technique using methanol as a solvent. Power level that used in the extraction is 280 W and the time range is between 10min to 50min at temperature range of 200C to 600C. After that the analysis was performed using High performance liquid chromatography (HPLC). Using 30 min, 40 0C and 80% methanol is the most suitable time, temperature and percent of solvent used to break the analyte and matrix bond and produce the steviol Glycoside in optimum yields. From this research we know that ultrasound extraction technique is most common technique and most suitable in order to extract the steviol glycosides from the mixture of s.rebaudiana stems and leaves. The optimum yield can afforded at extraction time of 30 minutes, extraction temperature of 40 0C and the percentage of methanol of 80%. Extraction kinetic of steviol glycosides approved the second-order kinetic yielding good R2 values of 0.997 and k values of 0.0301.
650 0 _aExtraction (Chemistry)
650 0 _aStevia
650 0 _aSweeteners
999 _aVIRTUA40
_c4016
_d4022
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*6502*9992