000 02307nam a2200253 a 4500
001 vtls000075722
003 KUKTEM
005 20251114204545.0
008 131023t2013 my da f abm 000 0 eng d
020 _aTHE0002233(Local)
039 9 _a201905241048
_bshamsul
_y201310231455
_znabilah
040 _aUMP
090 _aQK898.A57 B33 2013 rs Bc.
100 0 _aBadariah Mohd Nasir
245 1 4 _aThe effect of extraction parameters on antioxidant activity of Cosmos caudatus /
_cBadariah Mohd Nasir
260 _aKuantan, Pahang :
_bUMP,
_c2013
300 _axiii, 59 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
504 _aBibliography : p. 45-51
520 3 _aThe present of natural antioxidant in plant is well known and Cosmos caudatus is one of the plants that have high content of antioxidant. The objective of this study was to investigate the effect of operating conditions on extraction of total antioxidant compound (TAC) from C. caudatus. TAC was extracted using 70 % (v/v) acetone as the solvent for 4, 8 hour and 12 hours. C. caudatus was then extracted at different to solid to solvent ratio of 1:20, 1:25 and 1:30 (w/v). Finally, C. caudatus was extracted at different extraction temperature (60, 80 and 100 oC) for 30 min. The results show that the best extraction time to extract the TAC was after 8 hour extraction using solvent to sample ratio of 1:30 (w/v) and 66 oC of extraction temperature. The TAC obtained was 28.8 mg AAE/g with the corresponding of 10.1% of its antioxidant activity. However, when considered the extraction temperature, the highest value of TAC (19.5 mg AAE/g) was observed in extraction at 80o C after 30 minute and for temperature 60 and 100 oC the TAC were 16.4 and 17.1 mg AAE/g. In HPLC analysis using the ratio sample to solvent at 8 hour, the highest TAC was at 1:30 (2.368 mg AAE/g) and also for the extraction temperature 80 oC has highest TAC (1.84611 mg AAE/g). This trend was similar with the result obtained from DPPH assay where 1:30 (w/v) and at 80 oC extraction has highest TAC.
650 0 _aAntioxidants
650 0 _aCompositae
999 _aVIRTUA40
_c4078
_d4084
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992