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008 131031t2012 my da f m 000 0 eng d
020 _aTHE0002270(Local)
039 9 _a201905311507
_bnazirah
_c201311281654
_dnabilah
_y201310311611
_znabilah
040 _aUMP
090 _aQP752.O44 F37 2012 rs Bc.
100 0 _aNor Farahiyah Ghazali
245 1 0 _aProduction of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity /
_cNor Farahiyah Ghazali
260 _aKuantan, Pahang :
_bUMP,
_c2012
300 _axiii, 47 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2012
504 _aBibliography : p. 39-41
520 3 _aOmega-3 polyunsaturated fatty acid (PUFA) plays a vital role in a number of human health aspects substantially for the regulation of biological function, prevention and treatment of human diseases. Subsequently, humans cannot synthesize the omega-3 PUFA, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), thus, intake comes predominantly from dietary. Therefore, the microalgae was discovered as an alternative source of fatty acids compared to fish oil since it offers better purification essential and has no risk of chemical contamination. Moreover, it also acts as a carbon dioxide fixer by photosynthesis process in reducing the atmospheric carbon dioxide. There are major factors give affect for microalgae growth which is carbon source, irradiance level, temperature, nutrient availability and salinity. This study is carried out to investigate carbon dioxide effect in the specific range of 2%, 5%, 10% and 15% at flow rate of 0.2 L/min. Meanwhile, the irradiance levels are examined by using 31 µmol photons m-2 s-1, 82 µmol photons m-2 s-1 , 125 µmol photons m-2 s-1 and 156 µmol photons m-2 s-1 by applying one factor at time (OFAT) in maximizing the production of omega-3 fatty acids. The microalgae of Nannochloropsis sp. are cultured in f/2 medium with 300 ml working volume for 7 days of cultivation in an experimental system of photobioreactor. The omega-3 fatty acid compositions of Nannochloropsis sp. under different culture conditions are analyzed by lipid extraction and gas chromatography analysis in order to acquire the maximum amount of yield production. The results showed that the highest eicosapentaenoic acid productivity by Nannochloropsis sp. had been obtained at 2% of carbon dioxide aeration and 156 µmol photons m-2 s-1 which are 31.3121 mg/ml and 35.1339 mg/ml, respectively. The fatty acid accumulation of Nannochloropsis sp. could be prevailed to produce a high yield under their optimal conditions which are in maximal efficiency of carbon dioxide and high of light intensity.
650 0 _aOmega-3 fatty acids
999 _aVIRTUA40
_c4091
_d4097
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992