000 02352nam a2200253 a 4500
001 vtls000076087
003 KUKTEM
005 20251114204546.0
008 131113t2013 my da f m 000 0 eng d
020 _aTHE0002261(Local)
039 9 _a201905241130
_bshamsul
_y201311131239
_znabilah
040 _aUMP
090 _aQP609.S47 S93 2013 rs Bc.
100 0 _aSiti Syarrah Mohammad Hanapiah
245 1 0 _aStability and kinetic study of serine protease from exocarp of Benincasa Hispida /
_cSiti Syarrah Mohammad Hanapiah
260 _aKuantan, Pahang :
_bUMP,
_c2013
300 _axviii, 39 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
504 _aBibliography : p. xix-xxii
520 3 _aSerine protease is widely used in many industrial applications especially in food industry because of their wide range of good solubility, substrate specificity, activity over wide pH and temperature range and high stability under extreme conditions. Benincasa hispida contains serine protease that usually can be found in the sarcocarp or seeds. Therefore, exocarp of Benincasa hispida is used determine the presence of serine protease. This research objective was to investigate the effect of pH, temperature and stability of the enzyme activities and also the enzyme kinetic of serine protease. Serine protease from exocarp of Benincasa hispida is purified using ammonium sulfate precipitation, and its activity at different parameters were measured by using ultraviolet-visible spectroscopy (uv-vis) at wavelength 660nm. The activity was inhibited by trichloroacetic acid, CCl3 COOH. The optimum pH for their activity is in alkaline range (pH7 – 9), and the enzyme activity dropped at pH11. Meanwhile, the optimum temperature of serine protease is in the range 50oC to 60oC, where its activity started to slows down at 70oC. The serine protease had a Michaelis-Menten constant, KM of 0.05172 mg/ml whereas the maximum rate of reaction, VMAX is . Serine protease activity is determined by the amount of enzyme that hydrolyzed casein to produce 1.0 µmole of tyrosine per minute.
650 0 _aSerine proteinases
650 0 _aCucurbitaceae
999 _aVIRTUA40
_c4106
_d4112
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992