000 02742nam a2200253 a 4500
001 vtls000075068
003 KUKTEM
005 20251114204548.0
008 131007t2012 my da f m 000 0 eng d
020 _aTHE0002244(Local)
039 9 _a201905241112
_bshamsul
_c201311141159
_dnabilah
_c201310231016
_dnabilah
_y201310071359
_znabilah
040 _aUMP
090 _aQP601 .A84 2012 rs Bc.
100 0 _aNorashikin Zulkaple
245 1 0 _aPurification and characterization of papain enzyme from papaya /
_cNorashikin Zulkaple
260 _aKuantan, Pahang :
_bUMP,
_c2012
300 _axii, 45 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2012
504 _aBibliography : p. 40-42
520 3 _aPurification and characterization of papain enzyme isolated from unripe Carica papaya fruit is proposed by using expanded bed adsorption chromatography. Papain also known as cysteine protease is a type of industrial enzyme. The high request of this enzyme has encourage research involving the purification of papain, in the most efficient was by reducing cost and time span and increasing the purity and concentration and recovery ratio of the product. The expanded bed adsorption process was carried out using Fastline 10 column and Amberlite XAD7HP as the adsorbent. The latex of unripe papaya was diluted in buffer solution. Before starting the expanded bed adsorption operation, there are certain parameters were determined in order to achieve optimum conditions for the expanded bed adsorption process. The binding buffer and elution buffer that is suitable for each stage was determined in percentage of ethanol. After that, the expansion characteristic of adsorbent Amberlite XAD7HP was determined. Then, the dynamic binding capacity of the adsorption stage was studied. Accordingly to the parameters that have been determined, the expanded bed adsorption process was operated based on the condition that has been determined. From the batch adsorption in the binding stage and elution stage, it was found that 10% ethanol was suitable for binding stage and 90% ethanol was suitable for elution stage. The maximum adsorption capacity obtained is 13.14 μg and the velocity when H/H0 is two has the value of 2.6 x 10-6 mm/s. The dissociation constant,Kd in the Langmuir equation is 6.97 μg/ml. Profile for expanded bed adsorption process is plotted and the three stage of expanded bed adsorption process can be seen where the stages include binding stage, washing stage and elution stage.
650 0 _aPapain
650 0 _aPapaya
999 _aVIRTUA40
_c4158
_d4164
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992