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008 131022t2013 my da f abm 000 0 eng d
020 _aTHE0002137(Local)
039 9 _a201905131656
_byusri
_y201310221051
_znabilah
040 _aUMP
090 _aQD323 .F83 2013 rs Bc.
100 0 _aMuhammad Fuad Fikri Amir
245 1 0 _aEffect of different additives on the production of bacterial cellulose from pineapple waste /
_cMuhammad Fuad Fikri Amir
260 _aKuantan, Pahang :
_bUMP,
_c2013
300 _axv, 73 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
504 _aBibliography : p. 63-64
520 3 _aThe pineapple peel wastes contain high concentration of biodegradable organic material such as carbohydrate that can be utilized for the production of organic acid. With the goal of being economically competitive and overcome the problem of disposal of the waste, pineapple waste can be potentially used to enhance the production of bacterial cellulose. Bacterial cellulose is known as polysaccharide and usually it been used traditionally in food industry and the latest it is used as a material for medical application. In this research, bacterial cellulose was produced by Acetobacter xylinum from pineapple waste as a fermentation medium with three different types of additives that is sodium alginate, microcrystalline cellulose and carboxymethylcellulose. Hence, the purpose of this experiment is to investigate the effect of three different additives on the production of bacterial cellulose by Acetobacter xylinum. Generally, there are three major parts in completing the research on production of bacterial cellulose by Acetobacter xylinum. The first part is preparation of inoculum by Acetobacter xylinum strain. The inoculum of Acetobacter xylinum is mother culture where later on will be used for the next fermentation process for the synthesis of bacterial cellulose. In the second part, the experiment was proceed for bacterial cellulose synthesis. Firstly, the inoculum of Acetobacter xylinum was prepared in the HS-medium. After obtaining the optimum yield of bacterial celullose production with different parameter of pH medium, three different additives were used at various concentrations to enhanced bacterial cellulose production. All the medium fermentation was incubated 30°C for 3 days. The optimum pH value was determined by the weight of bacterial cellulose, where the medium fermentations were incubated for three days. The production bacterial cellulose was relatively high with similar properties to that produced in HS medium. Wet weight of bacterial cellulose production by Acetobacter xylinum without microcrystalline cellulose was 0.1097g, while bacterial cellulose production was the highest, 0.589 g, at 0.2% MCC. Besides that, the results show that these samples consist of ultrafine fibrils, which form the reticulated structure. The additions of additives into the medium does not effect the structure of the cellulose. These results suggest that it is possible to produce bacterial cellulose from low-cost resources in order to increase its production to a larger scale.
650 0 _aPineapple
650 0 _aBacteria
650 0 _aCellulose
999 _aVIRTUA40
_c4175
_d4181
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