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008 131007t2013 my da f m 000 0 eng d
020 _aTHE0002109(Local)
039 9 _a201905131640
_byusri
_c201311141204
_dnabilah
_c201310231023
_dnabilah
_y201310071644
_znabilah
040 _aUMP
090 _aQD272.C4 N35 2013 rs Bc.
100 0 _aWan Najibah Mohamad
245 1 0 _aPreparation of ion exchange membrane chromatography by modification of Polyethersulfone membrane through UV grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer /
_cWan Najibah Mohamad
260 _aKuantan, Pahang :
_bUMP,
_c2013
300 _axiii, 56 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2013
504 _aBibliography : p. 38-40
520 3 _aChromatographic separation of protein mixtures has become one of the most effective widely used means of techniques to purifying individual proteins. Packed bed chromatography is the common technique that is used configuration for the protein separation. However, packed bed chromatography has its some limitations during separation process such as high pressure drop and time consuming. Membrane chromatography then introduced to overcome the limitations of the packed bed chromatography. In the current research, polyethersulfone (PES) commercial membrane was converted into ion exchange (IEX) membrane chromatography by attaching [2-(acryloyloxy)ethyl] trimethyl ammonium chloride (AETMA) and acrylic acid (AA) monomer using UV light irradiation technique. The effect of AETMA and AA monomer concentration from 1.5 M to 2.0 M was studied. The IEX membrane was characterized in term of degree of grafting, changed of functional group as well as protein binding capacity using pure bovine serum albumin. For AETMA-grafted membrane, the binding capacity increase about the 65.32 % as the monomer concentration increase from 1.5 M to 2.0 M. While, for AA-grafted membrane, the binding capacity shows a huge increment when the monomer concentration was increase from 1.5 M to 2.0 M with amount 78.65 %.
650 0 _aChromatographic analysis
999 _aVIRTUA40
_c4190
_d4196
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992