000			02847nam a2200241 a 4500
001			vtls000075885
003			KUKTEM
005			20251114204550.0
008			131107t2013 my da f m 000 0 eng d
020			_aTHE0005056(Local)
039		9	_a201905140951 _bSHAHRILJ _c201311131645 _dnabilah _y201311071153 _znabilah
040			_aUMP
090			_aTX819.S75 Z85 2013 rs Bc.
100	0		_aZulkarnain Jema'at
245	1	0	_aIsolation of Steviol Glycosides from the mixtures of S.Rebaudiana stems and leaves using ethanol and ultrasound technique / _cby Zulkarnain Jema'at
260			_aKuantan, Pahang : _bUMP, _c2013
300			_axii, 38 p. : _bill. ; _c30 cm. + _e1 CD-ROM
502			_aProject paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2013
504			_aBibliography : p. 31-34
520	3		_aThe mixture of s.rebaudiana stems and leaves will be extracted to get the stevioside. Stevioside is a diterpene steviol Glycoside which is produced by conventional process. It is used to sweeten soft drink,soya sauce, yoghurt and other food. It also good for diabetic patients. This research objective is to extract the steviol glycosides from the mixture of s.rebaudiana stems and leaves using ultrasound extraction technique and find their applications. The process normally include the extraction, pre-treatment, separation, purification and refining. These processes will use ultrasound technique and followed by High performance liquid chromatography analysis (HPLC). Ultrasound extraction technique is one of the techniques to extract the mixture of s.rebaudiana stems and leaves before the sample of the the mixture of s.rebaudiana stems and leaves is analyzed by HPLC. The mixture of s.rebaudiana stems and leaves will be in dry and powder condition before it extracted by ultrasound extraction technique using ethanol as a solvent. Power level that used in the extraction is 280 W and the time range is between 10min to 50min at temperature range of 200C to 600C. After that the analysis was performed using High performance liquid chromatography (HPLC). Using 30 min, 40 0C and 80% ethanol is the most suitable time, temperature and percent of solvent used to break the analyte and matrix bond and produce the steviol Glycoside in optimum yields. From this research we know that ultrasound extraction technique is most common technique and most suitable in order to extract the steviol glycosides from the mixture of s.rebaudiana stems and leaves. The optimum yield can afforded at extraction time of 30 minutes, extraction temperature of 40 0C and the percentage of ethanol of 80%. Extraction kinetic of steviol glycosides approved the second-order kinetic yielding good R2 values of 0.992.
650		0	_aStevia
999			_aVIRTUA40 _c4202 _d4208
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992