000 02402nam a2200241 a 4500
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005 20251114204550.0
008 131008t2012 my da f m 000 0 eng d
020 _aTHE0002205(Local)
039 9 _a201905131718
_byusri
_c201311181656
_dnabilah
_c201310221704
_dnabilah
_y201310081219
_znabilah
040 _aUMP
090 _aQK495.C74 H39 2012 rs Bc.
100 0 _aNor Hayati Shahidan
245 1 0 _aExtraction of stevioside from Stevia Rebaudiana leaves using cellulase /
_cNor Hayati Shahidan
260 _aKuantan, Pahang :
_bUMP,
_c2012
300 _axiv, 49 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2012
504 _aBibliography : p. 38-42
520 3 _aStevioside is a diterpene glycoside present in Stevia Rebaudiana leaves that has the ability to sweeten at rated between 70 to 350 times than sucrose (0.4% w/v). It has no calorific value. Unlike many low calorie sweeteners, stevioside is stable at high temperature. The objective of this research is to extract stevioside from stevia rebaudiana leaves by using cellulase from Aspergillus Niger. Acetate buffer and ethanol were used as a medium for enzyme and as a solvent, respectively. In this present study, the enzymatic extraction of stevioside from stevia rebaudiana leaves was carried out using cellulase with various parameters that affect the production of stevioside such as concentration of enzyme, incubation time and temperature. Cellulase was observed to give the highest stevioside yield (16230 ± 0.3 μg/ml) at 40oC. This indicated that the maximum temperature for cellulase activity was 40oC. The results signify that the enzymatic extraction method is an alternative to solvent based stevioside extraction, based on its higher efficiency. Thus, it can be concluded that the extraction of stevioside from Stevia rebaudiana leaves using cellulase can be maximized under the maximum conditions for the cellulase activity where the used of solvent can be minimized in degrading the cell wall Together with the maximum heat and correct combination of the solvent used, a new and efficient way of extracting high yield of stevioside can be obtained.
650 0 _aStevia rebaudiana
999 _aVIRTUA40
_c4215
_d4221
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992