000			02925nam a2200253 a 4500
001			vtls000076099
003			KUKTEM
005			20251114204552.0
008			131114t2013 my da f m 000 0 eng d
020			_aTHE0004085(Local)
039		9	_a201905160924 _bfauzi _y201311141227 _znabilah
040			_aUMP
090			_aTP156.E8 S58 2013 rs Bc.
100	0		_aSharifah Shufiza Khaziri Tuan Hadi
245	1	0	_aPerformance of red pigment production by Monascus purpureus at different extraction methods / _cSharifah Shufiza Khaziri Tuan Hadi
260			_aKuantan, Pahang : _bUMP, _c2013
300			_axv, 59 p. : _bill. ; _c30 cm. + _e1 CD-ROM
502			_aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
504			_aBibliography : p. 50-52
520	3		_aThe purpose of this research is mainly to study the performance of red pigment production at different extraction methods. The strain used in this study is Monascus purpureus which produce red pigment and red yeast rice was used as the substrate in the solid state fermentation. The initial moisture content in fermentation would be 56% which was considered as optimal pigmentation to be occurred. It would then be inoculated with 1x107 spore/ml suspension of inoculum. Extraction methods including solvent extraction, Soxhlet extraction, agitated extraction and ultrasonic extraction were being carried out after fermentation process complete. Each methods required several conditions to be fulfilled to achieve the objective of this research. In this study, the best solvent used was 60% ethyl alcohol. Uv-Vis spectrophotometer is used to analyze red pigments in the extracts which were quantified by reading the absorbance at wavelength of 500 nm for this pigment. Thus, the best performance of the methods was identified by maximum extracts achieved which was measured by concentration of extracted product obtained. Solvent extraction was used as the reference and this method produced 1.278AU/g.d pigment extract. The results were 1.268AU/g.d for ultrasonic extraction, 1.265AU/g.d for agitated extraction and 0.128AU/g.d for Soxhlet extraction. The best method was ultrasonic extraction as compared to solvent extraction in a separate experiment and it produced 0.920AU/g.d than in solvent extraction, 0.869 AU/g.d pigment extract. The increrment percentage was about 5.81%. Besides, an analysis of variance (ANOVA) was carried out to investigate the significance to use the methods, showing the p-value. The p-value for agitated extraction was 3.63x10-7, 8.35x10-8 for ultrasonic extraction and 3.79x10-7 for Soxhlet extraction. All of the methods were significant to be carried out since all of the p-value was lower than 0.05.
650		0	_aExtraction (Chemistry)
650		0	_aMonascus purpureus
999			_aVIRTUA40 _c4257 _d4263
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992