000 02203nam a2200253 a 4500
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003 KUKTEM
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008 131113t2013 my da f m 000 0 eng d
020 _aTHE0002259(Local)
039 9 _a201905241128
_bshamsul
_c201311130846
_dnabilah
_y201311130844
_znabilah
040 _aUMP
090 _aQP609.P3 Y86 2013 rs Bc.
100 0 _aMuhammad Yunus Zainal Abidin
245 1 0 _aStability of papain in aqueous organic solvent by reverse phase liquid chromatography /
_cMuhammad Yunus Zainal Abidin
260 _aKuantan, Pahang :
_bUMP,
_c2013
300 _axiii, 49 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
504 _aBibliography : p. 37-38
520 3 _aPapain is one of the proteolytic enzymes found in the latex of the leaves and of the green fruit of the papaya tree (Carica papaya L.). It’s known to be highly used in the several industry like food, medical, brewing and many more. Hence, the objective of this research is to study the stability of papain in aqueous organic solvent and secondly to purify the papain using the AmberliteTM XAD7HP as the reverse phase liquid chromatography adsorbent. The research is based on experimental lab research. The effects of different concentrations of the water-miscible organic solvents acetonitrile and ethanol on the stability of papain in aqueous solution were studied. Batch adsorption using AmberliteTM XAD7HP was carried out to purify papain from papaya juice. The purified papain was analysed using the Bradford assay, the proteolytic papain activity assay and SDS PAGE. As for conclusion the of the study. The result obtained proved that papain has exhibited higher stability in aqueous acetonitrile than in ethanol under small margin. Decreases in the activity of the enzyme were observed at organic solvent concentrations above 60%. Papain with high purity was achieved by adsorption using the AmberliteTM XAD7HP.
650 0 _aPapain
650 0 _aLiquid chromatography
999 _aVIRTUA40
_c4306
_d4312
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992