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008 131113t2013 my da f m 000 0 eng d
020 _aTHE0004521(Local)
039 9 _a201905131527
_bazhar
_y201311130906
_znabilah
040 _aUMP
090 _aTP248.65.P76 A35 2013 rs Bc.
100 0 _aAhmad Afifi Ibrahim
245 1 0 _aEffect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis /
_cAhmad Afifi Ibrahim
260 _aKuantan, Pahang :
_bUMP,
_c2013
300 _axvi, 68 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
504 _aBibliography : p. 60-63
520 3 _aThe purpose of this study is to investigate the effect of pH and enzyme loading on the concentration of protein during enzymatic hydrolysis of Collocalia Fuciphaga. C. Fuciphaga is refers to the bird nest from one swiftlet species that is used as the sample known to be highly nutritious. Bovine Serum Albumin (BSA), a standard protein solution was used as the benchmark for the reference of the protein concentration. A research had been proposed in this study to optimize the production of protein from C.Fuciphaga. The effect of changing of the parameters values during the enzymatic hydrolysis was studied and the higher protein concentrations produced were highlighted. The standard protein curve of Bovine Serum Albumin (BSA) is prepared first with several dilutions for protein standard curve. The results from two significant parameters in the experiment was taken and analyzed. The selection of parameters values was taken from the enzymes’ optimum condition where the pH value of Alcalase 2.4L is from pH 7 until pH 10 whiles the temperature is fixed at 50oC. Enzyme loading was varies from 1.5% to 3.0% (v/w). The sample was analyzed using UV-Vis Spectrophotometer to determine the concentration of protein in each sample. The optimum condition is found to be at extraction condition of pH 8.5 and enzyme concentration of 3.0% (v/w). Characterization of the sample had been done and validated the protein composition on the extracted sample.
650 0 _aProteins
650 0 _aHydrolysis
999 _aVIRTUA40
_c4319
_d4325
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992