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008 131113t2013 my da f m 000 0 eng d
020 _aTHE0004756(Local)
039 9 _a201905141152
_bsmfh
_c201905141150
_dsmfh
_y201311131041
_znabilah
040 _aUMP
090 _aTP414 .F38 2013 rs Bc.
100 0 _aNoor Fauzani Ayop
245 1 0 _aEnzyme hydrolysis of old newspaper to produce simple sugar for fermentation process /
_cNoor Fauzani Ayop
260 _aKuantan, Pahang :
_bUMP,
_c2013
300 _axv, 64 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
504 _aBibliography : p. 41-43
520 3 _aProduction of fermentable sugar from lignocellulosic material was attempted by many researchers. Nowadays, this is because of plenty lignocellulosic biomass waste available around the world including Malaysia. Newspaper is a type of lignocellulosic sources because newspaper was made from softwood that contains cellulose, hemicelluloses and lignin. Cellulose component in newspaper is the major component with 45-50 %. The celluloses were able to be converted into simple sugar, which is glucose. Enzymatic hydrolysis is preferred for the conversion because less cost and high conversion. In this study, cellulase enzyme from Novozyme was used to hydrolyze newspaper for glucose production. By applying one-factor-at-time (OFAT), six parameters were being studied. They are surfactant ratio, enzyme loading, buffer pH, incubation temperature, agitation speed and incubation time. In all experiments, glucose production was monitored by DNS method. The result obtained show that at surfactant ratio 0.2:0.8 of TX-21: TWEEN-80, 1.0 ml of cellulase enzyme, at pH 6.0 when mixed with 0.2 g substrate at 50 oC and agitated at 200 rpm for 96 h is suitable for enzyme reaction. Those optimum parameters obtained gave the higher glucose production at 0.73 0.02 g/L.
650 0 _aGlucose
650 0 _aHydrolysis
650 0 _aEnzymes
999 _aVIRTUA40
_c4330
_d4336
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*6502*9992