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008 140521t2013 my a f m 000 0 eng d
020 _aTHE0004903(Local)
039 9 _a201905131252
_bSHAHRILJ
_c201710121012
_daishah
_c201405211615
_dFida
_y201405211614
_zFida
040 _aUMP
090 _aTP973 .A95 2013 Thesis
100 0 _aNik Nor Aziati Abd Aziz
245 1 0 _aOptimization of pre-treatment process of glycerol residue for succinic acid production /
_cNik Nor Aziati Abd Aziz
260 _aKuantan, Pahang :
_bUMP,
_c2013
300 _axx, 132 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 CD-ROM
502 _aThesis (Master of Engineering in Bio-Process) -- Universiti Malaysia Pahang - 2013
504 _aBibliography : p. 106-113
520 3 _aThe purpose of this study is to recover glycerol from the oleo-chemical industry waste through acid pre-treatment process. Pre-treatment intervals parameters (pH, temperature and mass substrate) was obtained from OFAT, while the optimum conditions for the recovery of glycerol obtained by using response surface methodology (RSM). Glycerol concentrations before and after pre-treatment and succinic acid derived from the fermentation process were analyzed using HPLC. FTIR spectrometry was used to identify functional groups of glycerol. The optimum conditions of pH, temperature and substrate mass for glycerol pre-treatment process is at 2.0, 35 ° C and 110.3600 grams respectively. In optimal conditions, the concentration of glycerol after pre-treatment process is 159.3120 g / L. Glycerol is then used in the fermentation by Escherichia coli K-12 types to produce succinic acid of 0.6698 g / L, with initial concentration of glycerol, rotational speed and temperature at 19.6700 g / L, 200 rpm and 37 ° C, respectively. Succinic acid production from glycerol derived from the recovery process is 8.67% lower than the production of succinic acid from commercial glycerol. Therefore, this study was able to demonstrate that the glycerol from the oleo-chemical industry waste can be used in the production of succinic acid.
650 0 _aGlycerin
856 4 0 _uhttp://ecollib.ump.edu.my/3879/
_zLibrary access only
999 _aVIRTUA40
_c4756
_d4762
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