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020 _aTHE0004132(Local)
039 9 _a201905132118
_bfarhana
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_dVLOAD
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_zfarina
040 _aUMP
090 _aTP156.F4 N67 2006 rs Thesis
100 0 _aNorhalmy B. Mohd Nor
245 1 0 _aProduction of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration /
_cNorhalmy B. Mohd Nor
260 _aKuantan, Pahang :
_bKUKTEM ,
_c2006
300 _axiii, 50 p. :
_bill. ;
_c30 cm. +
_e1 computer disc
502 _aProject paper (Bachelor of Chemical Engineering) -- Kolej Universiti Kejuruteraan dan Teknologi Malaysia - 2006
504 _aBibliography: p. 44-46
520 _aABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. The study is focus on the effect of substrate concentration which is 1 g/L, 3g/L and 5 g/L. All the enzymes were incubating in temperature 37 oC and pH 5.0 for 30 minutes. The reaction was quenched by cooling on ice and the amount of reducing sugar was measured by dinitrosalicyclic (DNS) acid procedure with slight modification (Miller, 1965). The mixture was then incubated at 100 oC for 5 minutes. Subsequently, the reaction was stopped by cooling on ice. Distilled water was added into the final volume of 16 ml and the color intensity was measured at wavelength of 575 nm. The concentration of SCB affected the xylanase and CMCase activity. Xylanase and carboxymethylcellulase activity was higher at lower concentration hen using 3 g of SCB
650 0 _aFermentation
650 0 _aSugarcane
999 _aVIRTUA
_c512
_d518
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992