000 03308ntm a2200277 a 4500
001 vtls000085192
003 KUKTEM
005 20251117113241.0
008 150203t2012 my a f m 000 0 eng d
020 _aTHE0004123(Local)
039 9 _a201905132106
_bfarhana
_c201710121529
_daishah
_y201502031149
_zfawwaz
040 _aUMP
090 _aTP156.F4 H53 2012 r Thesis
100 0 _aNor Hidawati Hj Elias
245 1 0 _aProduction of succinic acid from glycerol and glycerol waste /
_cNor Hidawati Hj Elias
260 _aKuantan, Pahang :
_bUMP,
_c2012
300 _axviii, 190 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
500 _aFaculty of Chemical & Natural Resources Engineering
502 _aThesis (Master of Engineering in Bioprocess) -- Universiti Malaysia Pahang – 2012
504 _aBibliography : p. 136-163
520 3 _aSuccinic acid is extensively used globally for manufacture of synthetic resin, biodegradable polymer, pharmaceutical product and food flavoring. Traditionally, succinic acid produced through petrochemical route. The process is non-renewable and will affected pollution in the environment. Therefore, more attention has been focused towards a friendly environment processes for succinic acid production by way of fermentation process; which is highly recommended nowadays. In this research, studies been done to produce succinic acid through anaerobic fermentation of glycerol waste using Escherichia coli and to investigate the effect of each factors that influence succinic acid production. The most influential parameter for succinic acid production obtained via preliminary study of parameter range selection were glycerol concentration, tryptone concentration, sodium carbonate (Na2CO3) concentration , inoculums density, pH and incubation period. This experiment has produced 0.973g/l succinic acid using One-factor-at-a-time (OFAT) approach from commercial glycerol sample. These six variables screened to select the most significant factor affected succinic acid production using fractional factorial design (FFD) based on first order model term. Four factors selected while, two factors eliminated for the next stage of experiment. Subsequently, full factor central composite designs (CCD) falling under RSM employed to develop a mathematical correlation model between the significant factors for physio-chemical variables of succinic acid production. The experiment has produced 7.55g/l succinic acid from commercial glycerol sample. Besides, a second order polynomial regression fitted and the model was adequate by R-squared 0.9864. Meanwhile, under the optimum level, it is about 7.34g/l succinic acid being produced from commercial glycerol and 7.32g/l of succinic acid produced from recovered glycerol. Overall, this research shows glycerine pitch can be used safely and effective as a carbon source for Escherichia coli to produce succinic acid. In addition, this research provides an exciting opportunity to transform low-grade glycerine pitch into highly demanded chemical such succinic acid
650 0 _aSuccinic acid
650 0 _aFermentation
856 4 0 _uhttp://ecollib.ump.edu.my/3898/
_zLibrary access only
999 _aVIRTUA40
_c5449
_d5455
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5000*5020*5040*5200*6500*6501*8560*9992