000 02859ntm a2200241 a 4500
001 vtls000083342
003 KUKTEM
005 20251117113245.0
008 141112t2013 my a f m 000 0 eng d
020 _aTHE0004078(Local)
039 9 _a201905160915
_bfauzi
_c201411261255
_dfawwaz
_c201411141252
_dfawwaz
_c201411121242
_dfawwaz
_y201411121239
_zfawwaz
040 _aUMP
090 _aTP156.E8 S27 2013 r Bc.
100 0 _aSiti Sarah Abdullah
245 1 0 _aOptimization of enzymatic extraction of stevioside from stevia rebaudiana leaves by using cellulase /
_cSiti Sarah Abdullah
260 _aKuantan, Pahang :
_bUMP,
_c2013
300 _axiv, 53 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology) -- Universiti Malaysia Pahang - 2013
504 _aBibliography : p. 45-48
520 3 _aStevioside is one of the components known as diterpene glycoside existing in Stevia rebaudiana leaves which have 250 to 300 sweeter than sucrose at the concentration of 0.4% (w/v). It is used as sweetening agents and taste modifier mostly in food industry. Moreover, stevioside has no calorific value and suitable used in therapeutic especially in treating diabetic people. The objective of this research is to optimize the extraction of stevioside from Stevia rebaudiana leaves by using the cellulase. A Central Composite Design (CCD) has been chosen to study the effect of enzyme concentration, incubation time and temperature on extraction of stevioside. The medium for enzyme used is acetate buffer while ethanol is applied as solvent in the enzymatic extraction of stevioside by performing various parameters such as concentration of enzyme, incubation time and temperature. The p-value for cellulase concentration and incubation time was less than 0.05 indicate that these factors have significant effects on the extraction yield of stevioside. The extraction of stevioside is represented by a quadratic model with p-value <0.0001. From the experimental work, an enzymatic extraction method gives the highest concentration of stevioside (840.839 μg/ml) at 45°C as maximum temperature, concentration of cellulase at 1.75% (w/v) and the incubation times of 105 minutes gives the maximum time required to complete the extraction process of stevioside. The extraction stevioside yield in this experiment gets more than predicted values (835.49μg/ml). However the experimental value is not much difference compare to the predicted value. The extraction of stevioside from Stevia rebaudiana leaves using cellulase is a new efficient way of obtaining high concentration of stevioside and can minimize the use of solvent and energy consumption in degrading the cell wall
650 0 _aExtraction (Chemistry)
999 _aVIRTUA40
_c5600
_d5606
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992