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020 _aTHE0004112(Local)
039 9 _a201905131408
_bfarhana
_c201712051548
_dhuda
_y201412221055
_zfawwaz
040 _aUMP
090 _aTP156.F4 A45 2014 r Bc.
100 0 _aNur Amira Maarof
245 1 0 _aProduction of poly (3-hydroxybutyrate) from pineapple waste /
_cNur Amira Maarof
260 _aKuantan, Pahang :
_bUMP,
_c2014
300 _axiv, 42 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
500 _aFaculty of Chemical & Natural Resources Engineering
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2014
504 _aBibliography : p. 29-32
520 3 _aP(3HB) is the potential replacement of synthetic plastics due to its biodegradability and sustainability. Pineapple waste is one of the example of food waste that capable to produce P(3HB) as they contains sucrose, glucose, fructose and other nutrients that essential for production of bio plastics. Thus, the aim of this research is to employ the sugar present in pineapple waste as the carbon source for the bacteria producing biopolymer. The objectives are as follow; to determine the sugar content in the pineapple waste juice, to study the effect percent volume of juice from pineapple waste on production of P(3HB), to study the effect of pH on P(3HB) production. The method involve in this study are cultivation of bacteria, preparation of pineapple waste, preparation of inoculum, P(3HB) biosynthesis in shake flask, cell dry weight analysis and analysis of P(3HB) using HPLC. The results showed that glucose content in the mixture of skins and pulps is the highest with total of 28.80 g/L compared to fructose and sucrose. Fructose content come second with 21.25 g/L while sucrose has the lowest content in the PWJ with 20.28 g/L. The pineapple waste juice (PWJ) is then used as medium for the production of P(3HB). The fermentation medium with 50% and 100% volume of PWJ has the highest concentration of P(3HB) of 2.01 g/L (30th hour) and 1.49 g/L (44th hour) respectively. As the total dry mass production, the medium with 75% of PWJ has the highest concentration of P(3HB) with 14.42 g/L. The total concentration of P(3HB) for 50% and 100% PWJ are 10.52 g/L and 4.42 g/L respectively. The total average concentration of P(3HB) produced in the medium at pH 7 is 8.97 g/L. The highest concentration was found to be at the 44th hours with 2.15 g/L. As conclusion, the medium that consists of 75% PWJ and 25% ultra-pure water with the mixture of nutrient rich element at pH 7 is the most suitable medium to produce P(3HB)
650 0 _aBiopolymers
650 0 _aFermentations
856 4 0 _uhttp://ecollib.ump.edu.my/9807/
_zAccess in library only
999 _aVIRTUA40
_c5648
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