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000			03575ntm a2200277 a 4500
001			vtls000084601
003			KUKTEM
005			20251117113248.0
008			141222t2014 my a f m 000 0 eng d
020			_aTHE0004715(Local)
039		9	_a201905131619 _bsmfh _c201712051625 _dhuda _y201412221121 _zfawwaz
040			_aUMP
090			_aTP363 .A36 2014 r Bc.
100	0		_aNurul Ain Jumri
245	1	0	_aEffect of extraction conditions on protein concentration during edible bird’s nest alkaline hydrolysis / _cNurul Ain Jumri
260			_aKuantan, Pahang : _bUMP, _c2014
300			_axvi, 106 p. : _bill. ; _c30 cm. + _e1 CD-ROM
500			_aFaculty of Chemical & Natural Resources Engineering
502			_aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2014
504			_aBibliography : p. 100-106
520	3		_aEdible bird’s nest (EBN) is originated from the saliva of swiftlet. There are four species of swiftlet which are Collocalia fuciphaga, Collocalia germanis, Collocalia maxima and Collocalia unicolor. Collocalia fuciphaga is the common species which can be found in Malaysia. EBN consists mainly of amino acids, carbohydrates and mineral salts but it major ingredients were protein. Protein from EBN can be extracted by water, acid, alkaline and enzymatic hydrolysis method. This study focused on alkaline hydrolysis using sodium hydroxide (NaOH) at 50°C. Prior to alkaline hydrolysis, pre-treatment method (EBN cleaning by soaked with water, feathers removal and fine plumage from EBN sample) has been carried out. The effect of extraction time, NaOH concentration and liquid solid ratio (LSR) on protein concentration have been determined during alkaline hydrolysis. The optimum condition of alkaline hydrolysis has been identified using response surface methodology (RSM). From the experimental result, it showed that the maximum protein concentration was at 25 minutes of extraction time, 30:1 of LSR and 100 g/L NaOH concentration. However, increased in LSR and NaOH concentration were increased the protein concentration. The optimum condition of protein concentration was 1.260 g/L during alkaline hydrolysis using RSM were found at 25 minutes of extraction time, 100g/L NaOH concentration and 30:1 of LSR. From the ANOVA result, the significant factor was extraction time (p=0.0187) and was clearly showed that further increased in extraction time (25 minutes) resulted in decreased in protein concentration due to the degradation of amino acid structure at long duration of extraction time. The FTIR spectrums of the untreated and treated sample resulted in the different trend of spectrum. In the treated sample after pre-treatment process and alkaline hydrolysis, it is showed that N-H and C=O stretching peak is increased. Besides, it was confirmed that the material was correspond to amides as it consists of C=O stretch. While, from the analysis using the ICP-MS, amount of cadmium, arsenic and lead in the sample was 0.002mg/L, 0.00006mg/L and 0.262mg/L respectively after pre-treatment. It also showed that percentage decreasing of argentums, cadmium and magnesium is the highest compared to others metal which were 99%, 97.70% and 97.24% respectively. It can be concluded that EBN can be performed as an alternative source for protein
650		0	_aProtein _xDenaturation
650		0	_aBirds _xNests
856	4	0	_uhttp://ecollib.ump.edu.my/9754/ _zAccess in library only
999			_aVIRTUA40 _c5672 _d5678
999			_aVTLSSORT008002000400090010002450260030005000502050405200650065018560*9992