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008 141222t2014 my a f m 000 0 eng d
020 _aTHE0004472(Local)
039 9 _a201905161616
_brohana
_c201712051633
_dhuda
_y201412221727
_zfawwaz
040 _aUMP
090 _aTP248.27.M53 A43 2014 r Bc.
100 0 _aNurul Amalina Alias
245 1 0 _aKinetic study on poly(3-hydroxybutyrate) production from oil palm frond juice in batch fermentation /
_cNurul Amalina Alias
260 _aKuantan, Pahang :
_bUMP,
_c2014
300 _axiii, 48 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
500 _aFaculty of Chemical & Natural Resources Engineering
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2014
504 _aBibliography : p. 19-21
520 3 _aThis research is about the kinetic study on poly(3-hydroxybutyrate), P(3HB) production from oil palm frond juice in batch fermentation. Batch fermentation experiment was conducted in 500 mL shake flasks by using Cupriavidus necator in order to produce P(3HB). Fresh oil palm frond (OPF) was pressed and undergoes certain processes such as filtration, centrifugation and sterilization to get oil palm frond juice. OPF juice were used as the fermentation substrate for P(3HB) production. In this study, the microbial growth (μ, μmax, YX/S, Ks) and product (YP/S, YP/X) kinetic parameter can be obtained by using MATLAB R2009b software. The models were fit to the data using Runge-Kutta 4th order Method by minimizing the error between experimental and predicted data using Simplex Method. Logistic Model was developed for the biomass growth model. Model for polyhydroxyalkanoates (PHA) was developed based on assumptions that each cell contains the same amount of PHA and it degrades at the same rate. Predicted data was obtained using function ode45 in MATLAB R2009b software which implements Runge-Kutta 4th Order Method. Based on the results obtained, it shows that the predicted data of biomass profile was fitted well to the experimental data but for PHB profile, the predicted data deviated quite far from the experimental data. This shown that the models developed were representing biomass growth very well. Kinetic parameters for microbial growth and production of P(3HB) were determined by using Monod equation. The values are = 0.0346h-1, Ks = 0.0930g/L, = 0.0344h-1, Yx/s= 0.4731 and = 0.0416
650 0 _aFermentation
650 0 _aMicrobial biotechnology
856 4 0 _uhttp://ecollib.ump.edu.my/18332/
_zAccess in library only
999 _aVIRTUA40
_c5814
_d5820
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5000*5020*5040*5200*6500*6501*8560*9992