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020 _a1588293793 (alk. paper)
020 _a9781588293794
020 _a1597450057 (e-ISBN)
039 9 _a201204161235
_bida
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_dVLOAD
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040 _aUMP
090 _aQH442.2 .N84 2006
245 0 0 _aNuclear reprogramming :
_bmethods and protocols /
_cedited by Steve Pells
260 _aTotowa, N.J. :
_bHumana Press,
_cc2006
300 _axiv, 333 p. :
_bill. (some col.) ;
_c24 cm.
490 1 _aMethods in molecular biology ;
_v325
504 _aIncludes bibliographical references and index
505 0 _aNuclear transplantation in xenopus / John B. Gurdon -- Nuclear transfer in sheep / William A. Ritchie -- Protocols for nuclear transfer in mice / Shaorong Gao -- Isolation of stromal stem cells from human adipose tissue / Andrew C. Boquest ... [et al.] -- Nuclear reprogramming by cell fusion / Robert H. Broyles ... [et al.] -- Polyethylene glycol-mediated cell fusion / Jian Yang and Ming Hong Shen -- Epigenetic reprogramming of somatic genomes by electrofusion with embryonic stem cells / Masako Tada and Takashi Tada -- Quantification of cell fusion by flow cytometry / Stephen Sullivan ... [et al.] -- Modulation of cell fate using nuclear and cytoplasmic extracts / Anne-Mari Håkelien, Kristine G. Gaustad, and Philippe Collas -- Transgenic systems in nuclear reprogramming / Megan Munsie, Peter Mountford, and Jennifer Nichols -- Using immunofluorescence to observe methylation changes in mammalian preimplantation embryos / Fátima Santos and Wendy Dean -- Observing s-phase dynamic of histone modifications with fluorescently labeled antibodies / Rong Wu, Anna V. Terry, and David M. Gilbert -- Quantitative analysis of telomerase activity and telomere length in domestic animal clones / Dean H. Betts ... [et al.] --
505 0 _aPluripotency: capacity for in vitro differentiation of undifferentiated embryonic stem cells / Cornelia Wiese ... [et al.] -- Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens / Bruce A. Fenderson ... [et al.] -- Analysis of the nucleolar compartment of the nucleus as an indicator of nuclear reprogramming after nuclear transfer / Jacques-E. Fléchon -- Methylation-sensitive polymerase chain reaction / Hannah R. Moore, Richard R. Meehan, and Lorraine E. Young -- Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis / Yong-Mahn Han, Seok-Ho Kim, and Yong-Kook Kang -- Chromatin immunoprecipitation assay for mammalian tissues / Fiona B. Turner, Wang L. Cheung, and Peter Cheung -- Histone modifications and transcription factor binding on chromatin: CHIP/PCR assays / Jaejoon Won and Tae Kook Kim -- In vivo genomic footprinting using LM-PCR methods / Hiromi Tagoh, Peter N. Cockerill, and Constanze Bonifer -- Analyzing histone modification using crosslinked chromatin treated with micrococcal nuclease / Pascal Lefevre and Constanze Bonifer
650 0 _aCell nuclei
_xTransplantation
_vLaboratory methods
700 1 _aPells, Steve
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_vv. 325
856 4 1 _3Table of contents
_uhttp://www.loc.gov/catdir/toc/ecip0516/2005020464.html
999 _aVIRTUA40
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