000			03072nam a2200277 a 4500
001			vtls000053390
003			KUKTEM
005			20251117150522.0
008			110531t2011 nyua f 001 0 eng d
020			_a9781607619123
039		9	_a201110180944 _basmadi _c201107140051 _dVLOAD _y201105311551 _zirma
040			_aUMP
090			_aQP550 .P76 2011
245	0	0	_aProtein chromatography : _bmethods and protocols / _cedited by Dermot Walls and Sinéad T. Loughran
260			_aNew York, NY : _bSpringer, _c2011
300			_ax, 527 p. : _bill. (some col.) ; _c27 cm.
490	1		_aMethods in molecular biology, _x1940-6029 ; _vv. 681
504			_aIncludes bibliographical references and index
505	0		_aA digest of protein purification -- Gel-filtration chromatography -- Immunoaffinity chromatography -- Avoiding proteolysis during protein chromatography -- Scale-up of protein purification : Downstream processing issues -- Phage display : A powerful technology for the generation of high specificity affinity reagents from alternative immune sources -- Engineering protein stability -- Microfluidics in protein chromatography -- Tagging recombinant proteins to enhance solubility and aid purification -- Storage and lyophilisation of pure proteins -- Differential precipitation and solubilization of proteins -- Ion-exchange chromatography : Basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase -- Protein quantitation and analysis of purity -- Purification of proteins fused to glutathione S-transferase -- Purification of proteins fused to maltose-binding protein -- Purification of proteins from baculovirus-infected insect cells -- Purification of poly-histidine-tagged proteins -- Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by cid/etd tandem mass spectrometry -- Tag removal by site-specific cleavage of recombinant fusion proteins -- Purification of antibodies using affinity chromatography -- Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens -- Measuring protein-protein interactions using biacore -- Ultra-performance liquid chromatography-mass spectrometry of proteins -- Hydrophobic interaction chromatography -- Fast protein liquid chromatography -- Shotgun proteomics : A qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS -- Shotgun proteomics : A relative quantitative approach using off-gel electrophoresis and LC-MS/MS -- Clinical proteomics : Liquid chromatography-mass spectrometry purification systems -- Strategies for the purification of membrane proteins -- A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases
650		0	_aProteomics
650		0	_aProteins _xAnalysis
650		0	_aChromatographic analysis
700	1		_aWalls, Dermot
700	1		_aLoughran, Sinéad T.
999			_aVIRTUA40 _c59971 _d59977
999			_aVTLSSORT0080*0200*0400*0900*2450*2600*3000*4900*5040*5050*6500*6501*6502*7000*7001*9991