| 000 | 02452ntm a2200265 a 4500 | ||
|---|---|---|---|
| 001 | vtls000091315 | ||
| 003 | KUKTEM | ||
| 005 | 20251117113305.0 | ||
| 008 | 150923t2015 my a f m 000 0 eng d | ||
| 020 | _aTHE0004463(Local) | ||
| 039 | 9 |
_a201905161558 _brohana _c201711281344 _dsaini _y201509231122 _zhuda |
|
| 040 | _aUMP | ||
| 090 | _aTP248.27.F86 L38 2015 r Bc. | ||
| 100 | 1 | _aLau, Cindy Chin Yee | |
| 245 | 1 | 0 |
_aReusability and leakage of immobilized laccase enzyme / _cCindy Lau Chin Yee |
| 260 |
_aKuantan, Pahang : _bUMP, _c2015 |
||
| 300 |
_avi, 35 p. : _bill. (some col.) ; _c30 cm. + _e1 CD ROM |
||
| 500 | _aFaculty of Chemical & Natural Resources Engineering | ||
| 502 | _aProject paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2015 | ||
| 504 | _aBibliography : p. 27-32 | ||
| 520 | 3 | _aLaccase (EC 1.10.3.2, benzenediol: oxygen oxidoreductase), a copper containing enzyme which can catalyse the oxidation of various organic and inorganic substrates, is usually used to decolorize the wastewater effluent and render phenolic compounds to less toxic component. The objective of this research is to compare the reusability and leakage between entrapped and covalently bonded laccase enzymes. The reusability of immobilized laccase enzyme was studied by reacting a batch of immobilized laccase enzymes with ABTS repeatedly for 15 cycles. The study of the leakage of immobilized laccase enzyme was carried out by storing the immobilized laccase enzymes in acetate buffer solution for 32 days. The acetate buffer solution samples were taken and reacted with ABTS. The samples reacted with ABTS were analysed using a UV-Vis spectrophotometer at 415 nm. The absorbance readings were recorded and enzyme activities were calculated. The data collected showed that the retained enzyme activities of entrapped and covalently bonded enzyme after being reused for 15 cycles are 33.50% and 48.19% respectively. On the other hand, the leakages of entrapped and covalently bonded laccase enzyme after 32 days are 13.9 % and 14.46 % respectively. In conclusion, the covalently bonded laccase enzymes are more stable in terms of reusability and storage stability compared to entrapped laccase enzymes. | |
| 650 | 0 |
_aLignin _xBiodegradation |
|
| 856 | 4 | 0 |
_uhttp://ecollib.ump.edu.my/id/eprint/9495 _zAccess in library only |
| 999 |
_aVIRTUA40 _c6181 _d6187 |
||
| 999 | _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5000*5020*5040*5200*6500*8560*9992 | ||