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000			04254ntm a2200277 a 4500
001			vtls000094028
003			KUKTEM
005			20251117113319.0
008			160321t2015 my a f 000 0 eng d
020			_aTHE0000166(Local)
039		9	_a201905311454 _bnazirah _c201603211259 _dshima _y201603211225 _zshima
040			_aUMP
090			_aFIST . M84 2015 r Thesis
100	0		_aMuhammad Khan
245	1		_aAntioxidant and anti-acetylcholinesterase potential from waste of selected solanaceous plants : isolation and identification of the active compund / _cMuhammad Khan
260			_aKuantan, Pahang : _bUMP, _c2015
300			_axvii, 111 p. : _bill. (some col.) ; _c30 cm. + _e1 CD-ROM
500			_aFaculty of Industrial Science and Technology
502			_aThesis (Doctor of Philosophy (Biotechnology)) -- Universiti Malaysia Pahang – 2015
504			_aBibliography : p. 76-88
520			_aAlzheimer‘s disease (AD) is one of the most common forms of dementia, affecting the elderly population globally. The occurrence of the ailment is on the rise; one person isbeing effected every 71 seconds. This rate is higher than many other fatal diseases, e.g., cancer, stroke, and heart failures. Therefore, searching for compounds with minimal side effects along with significant activity against acetylcholinesterase (AChE), are needed to be discovered to fight AD. In order to achieve this goal the study was undertaken to screen 32 medicinal plants based on their total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant activity (AOA), and antiacetylcholinesterase activity (AChE) to select the best source of the active compounds that can be used for the treatment of Alzheimer‘s disease. For the purpose, crude extracts from all 32 plants were subjected to determine TPC, TFC, AOA, and antiAChE activity, and 3 plants out of 32 were finally selected for further study. The selected plants include Solanum tuberosum (Potato), Solanum melongena (Brinjals), andCapsicum annuum (Chilli). All three species belong to genus solanum and family solanaceae. TPC was determined following the Folin-Ciocalteu colorimetric method andTFC was determined using aluminium chloride colorimetric assay. AOA was determined by DPPH-scavenging and β-carotene assay methods, while anti-AChE activity was assayed by Ellman‘s method. A pure compound α-solanine was isolated by TLC, followed by HPLC and prep-HPLC. The fractions and isolated compound both showed significant bioactivities. The crude extract of S. tuberosum showed the highestanti-AChE activity (IC50= 689.9 μg/mL), followed by, S. melongena (IC50= 731.99 μg/mL), and C. annuum (IC50= 851 μg/mL). Purified α-solanine demonstrated the second highest anti-AchE activity (IC50= 725.70 μg/mL). A similar trend was seen for their antioxidant activity. In this case, TPC and TFC showed significant correlation with AOA and anti-AchE activity; attributing the bioactivities to be due to phenolic and flavonoids compounds present in plant fractions. The correlation between AChE,DPPH, TPC and TFC, were all found to be statistically significant (P<0.05). Furthermore, the finding shows that a good antioxidant compound can be a potent inhibitor of AChE. The data revealed that S. tuberosum fraction had higher activitycompared to α- solanine that might be due to the synergistic effect of alkaloidal compounds and the polyphenols present in the crude extract. The structure of the isolated compound was elucidated by different chromatographic and spectroscopic techniques including HPLC, FTIR, NMR and LC-MS. The research results indicated that Solanum species could be the candidates of choice in further search for new AChE inhibitors.
610	2	0	_aFaculty of Industrial Science and Technology _xDissertations
650		0	_aUniversities and Colleges _xDissertations
650		0	_aTheses
999			_aVIRTUA40 _c6572 _d6578
999			_aVTLSSORT008002000400090010002450260030005000502050405200610065006501*9992