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008 160316t2015 my a f 000 0 eng d
020 _aTHE0000176(Local)
039 9 _a201905131632
_bnazirah
_c201710111629
_daishah
_y201603161105
_zhuda
040 _aUMP
090 _aFIST .N35 2015 r Thesis
100 0 _aNailah Sa’adah
245 1 _aApplication of enzyme-producing bacteria for municipal solid waste biodegradation /
_cNailah Sa’adah
260 _aKuantan, Pahang :
_bUMP,
_c2015
300 _axvi, 96 p. :
_bill. ;
_c30 cm. +
_e1 CD-ROM
500 _aFaculty of Industrial Science and Technology
502 _aThesis (Master of Science (Biotechnology)) -- Universiti Malaysia Pahang – 2015
504 _aBibliography : p. 82-95
520 3 _aPopulation growth with increasing consumption levels leads to abundant waste in Kuantan. Jabor landfill, commonly known as Kuantan landfill, receives more than 500 tons of waste per day with a composition of 60% of domestic waste and 40% of commercial waste. The composition of domestic waste were organic waste, green waste, mixed paper, plastic, textile, ferrous, glass, rubber and leather, and others. Meanwhile, the compositions of commercial waste are food, plastic, yard waste, paper, cardboard, textile, glass, non-ferrous, rubber, wood, ferrous metals. Landfill system always produces leachate. This waste contains many types of bacteria with the potential to degrade the waste compound. Screening tests with selective media demonstrated the ability of bacteria to produce amylase, lipase, protease and cellulase enzymes. These enzymes are needed to accelerate the molecules breakdown of municipal solid waste in the biodegradation process. Samples for isolation of bacteria were taken from different places, namely landfill soil and leachate. Identification of bacteria was conducted using Gen III microplate BIOLOG microbial identification system. They were Bacillus amyloliquefaciens, Bacillus ruris, Bacillus licheniformis, Bacillus subtilis and Kocuria varians. There were 4 different treatments: composting pile wit hout bacteria (T0), composting pile with inoculation of amylase and protease producing bacteria (T1),composting with inoculation of lipase and cellulase producing bacteria (T2), and composting with inoculation all enzyme-producing bacteria (T3). The result of biodegradation process of T3 treatment reached the highest temperature (53° C) with the longest thermophilic phase compared to other treatments. The lower value of C/N ratio, the more stable the level of maturity of compost. The lowest of C/N ratio value was T3 (10%). T3 treatment compared with other treatments can increase as much as 27% content of nitrogen, 67% of phosphorus and 33% of potassium. All the treatments with bacterial inoculation (T1, T2, and T3) are able to reduce the content of heavy metals (Fe, Zn, Cu) on municipal solid waste biodegradation. It can be concluded that the inoculation of potential enzyme-producing of bacteria on municipal solid waste biodegradation is effective to increase the nutrient content and decrease the heavy metals.
610 2 0 _aFaculty of Industrial Science and Technology
_xDissertations
650 0 _aUniversities and Colleges
_xDissertations
650 0 _aTheses
856 4 0 _uhttp://ecollib.ump.edu.my/3553/
_zLibrary access only
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