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008 180312s2017 my da f am 000 0 eng d
020 _aTHE0001365(Local)
039 9 _a201905211117
_basmadi
_y201803121632
_zsaini
040 _aUMP
_beng
_cUMP
_erda
090 _aFTeK .J37 2017 r Thesis
100 1 _aJarial, Rini,
_eauthor.
245 1 0 _aExtraction, characterization and purification of flavonoids from filicophytes /
_cRini Jarial
264 1 _aKuantan, Pahang :
_bUMP,
_c2017
264 4 _c© 2017
300 _axiii, 126 pages :
_billustrations (some color), chart ;
_c30 cm. +
_e1 CD-ROM
336 _atext
_2rdacontent
336 _atext
_2rdacontent
337 _aunmediated
_2rdamedia
337 _acomputer
_2rdamedia
338 _avolume
_2rdacarrier
338 _acomputer disc
_2rdacarrier
347 _atext file
_bPDF
_2rda
500 _aFaculty of Engineering Technology
502 _aThesis (Doctor of Philosophy) -- Universiti Malaysia Pahang – 2017
504 _aIncludes bibliographical references
520 3 _aPlants are rich source of phytochemicals (flavonoids, terpenoids, alkaloids, phenols). These natural products have played a key role in pharma research, as many medicines are either natural products or derivatives. The present study is the exploration of selected ferns (Drynaria rigidula, Chelianthes tenuifolia, Asplenium nidus, Dipteris conjugata and Antrophyum callifolium) as a new candidate of natural compounds. This was done through a series of plant extraction, characterization and purification protocols. The methanolic fern-extracts (MFEs) from all five ferns were subjected to determine total phenolic content (TPC), total flavonoid content (TFC), antibacterial and antioxidant activities. TPC was determined following the Folin-Ciocalteu colorimetric method and TFC was determined using aluminium chloride colorimetric assay. Highest amount of TFC was noted in C. tenuifolia whereas best TPC was in D. rigidula. The MFEs of these ferns were assessed for antibacterial and antifungal activities by measuring inhibition zones against a panel of pathogenic bacterial and fungal strains using agar well diffusion method. The C. tenuifolia showed maximum inhibition zone against Staphylococcus aureus (26 mm). Out of all ferns, C. tenuifolia showed a significant antioxidative activity in vitro i.e. 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging potential of C. tenuifolia (MFE) with IC50 value of 50.37 mM. However, for human hepatoma HepG2 and human carcinoma HeLa cells C. tenuifolia (MFE) exhibited high anticancer activity using 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide (MTT) assay than others ferns. The data revealed that MFE of C. tenuifolia had higher antioxidant and antibacterial activities that might be due to the polyphenols present in the crude extract. Among the five ferns we selected the best fern (C. tenuifolia) for purification of flavonoids. Two flavonoids were identified as rutin (2.8 mg) and quercetin (3.34 mg). The MIC values of purified flavonoids were 2.25 and 0.45 μg/ml against S. aureus and Enterobacter sp., respectively. Rutin and quercetin showed significant in vitro antioxidant activity, the scavenging potential of quercetin (86.1%) was higher than that of rutin (73.2%) were measured against stable, non –biological radicals such as DPPH. However, for human hepatoma HepG2 and human carcinoma HeLa cells, quercetin (80.91 ± 1.93) exhibited high anticancer activity than rutin (11.10 ± 2.10). The results suggested that MFE of C. tenuifolia could potentially be employed in traditional medicine as they are rich in compounds with antioxidant, antimicrobial and anticancer properties.
610 2 0 _aFaculty of Engineering Technology
_xDissertations
650 0 _aUniversities and colleges
_xDisertations
650 0 _aTheses
999 _aVIRTUA40
_c7332
_d7338
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2640*2641*3000*3360*3361*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992