000 03890ntm a2200373 i 4500
001 vtls000103163
003 KUKTEM
005 20251117113357.0
008 180326s2017 my da f am 000 0 eng d
020 _aTHE0005460(Local)
039 9 _a201905151726
_bhanafiah
_y201803261032
_zsaini
040 _aUMP
_beng
_cUMP
_erda
090 _aPBMSK .H53 2017 r Thesis
100 0 _aTengku Nor Hidayati Tengku Zainal Abidin,
_eauthor.
245 1 0 _aKaedah al-istibrā' haiwan akuatik menurut perspektif Islam dan sains akuakultur :
_bpangasius sutchii /
_cTengku Nor Hidayati Tengku Zainal Abidin
264 1 _aKuantan, Pahang :
_bUMP,
_c2017
264 4 _c© 2017
300 _axvii, 111 pages :
_billustrations (some color), charts ;
_c30 cm. +
_e1 CD-ROM
336 _atext
_2rdacontent
336 _atext
_2rdacontent
337 _aunmediated
_2rdamedia
337 _acomputer
_2rdamedia
338 _avolume
_2rdacarrier
338 _acomputer disc
_2rdacarrier
347 _atext file
_bPDF
_2rda
500 _aCentre for Modern Languages and Human Sciences
502 _aThesis (Master of Science in Halal Food) -- Universiti Malaysia Pahang – 2017
504 _aIncludes bibliographical references
520 3 _aHalal is the Islamic term that refers to something lawful or permissible. As such, undoubtedly halal animal can be consumed by Muslim once slaughtered properly according to Islamic teaching. However, in the case of halal animals that fed with contaminated food/filth (najis) continuously, the status of the animal according to majority of the scholars, is changed to al-jallālah. Animals fed with najasa are categorized as al-jallālah (contaminated animals) are only allowed to be consumed if they are quarantined for a certain period of time. Duration of quarantine varies according to the type of animals, that between one to forty days. During this quarantine period the animals will undergo a natural purification process or istiḥālah. Patin fish (pangasius sutchii) that is commonly consumed in Malaysia could be fed on najasa.like pig organs. During the quarantine or al-istibrā', natural purification process/istiḥālah will take place in fish digestive system. This study was carried out to estimate the istiḥālah period for patin fish after feeding with pig offal, based on the absence of DNA porcine in patin fish stomach and to suggest the sufficient period/time of istiḥālah for patin fish. Quarantine period is determined when there is completely no traces of DNA porcine from the fish. Detection of DNA porcine was done by using PCR and RT-PCR. The findings show detection of DNA porcine in fish stomach that fed pig organs, while DNA porcine was not detected on the skin and fish fillet during zero (0) hour quarantine period. After the 20 hours quarantine, DNA porcine was not detected in the stomach of the fish. The volume of water used varies between (20%, 50%, and 80%) for purification of patin from pig contamination. DNA porcine was not detected in the stomach of fish which were quarantined for 24 hours using the volume of water 20%, 50%, and 80%. Thus, the al-istibra‟ method can solve the issue of al-jallālah animals and further enhance the Malaysia aquaculture industry. Extraction of genomic DNA from tissue of different fish part, water and pig offal with the application of RNase A, improved the quality of DNA obtainded for most of molecular application based on the DNA purity. The total DNA yield is different among the sample this might be due to the different structure and compactness of the fish tissue.
610 2 0 _aCentre for Modern Languages and Human Sciences
_xDissertations
650 0 _aUniversities and colleges
_xDisertations
650 0 _aTheses
999 _aVIRTUA40
_c7619
_d7625
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2640*2641*3000*3360*3361*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992