000 03072ntm a2200277 a 4500
001 vtls000101453
003 KUKTEM
005 20251117113358.0
008 171002s2017 my a f am 000 0 eng d
020 _a101453
039 9 _a201905141007
_bnazirah
_c201710021610
_dsaini
_y201710021603
_zsaini
040 _aUMP
090 _aFIST .R69 2017 r Thesis
100 0 _aRoziasyahira Mutazah
245 1 0 _aChemical constituents, antioxidant and cytotoxicity properties of Rhodomyrtus Tomentosa root extracts /
_cRoziasyahira Mutazah
260 _aKuantan, Pahang :
_bUMP,
_c2017
300 _axiv, 107 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 CD-ROM
500 _aFaculty of Industrial Sciences and Technology
502 _aThesis (Master of Science) -- Universiti Malaysia Pahang – 2017
504 _aBibliography : p. 66-78
520 3 _aA study was carried out on the chemical constituents, antioxidant and cytotoxicity properties of Rhodomyrtus tomentosa root extracts. Solvent–solvent extraction, fractionation and separation using different chromatographic techniques (column chromatography and thin layer chromatography) were used for isolation of pure compounds. The chemical constituents of the roots of R. tomentosa were subjected to ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-QToF/MS) for the determination of compound composition present in the plant. The potential cytotoxicity of R. tomentosa extracts from different solvents were evaluated in vitro on HepG2, MCF-7 and HT 29 cell lines and antioxidant activity was monitored by radical scavenging assay (DPPH), copper reducing antioxidant capacity (CUPRAC) and β-carotene bleaching assay. Each extract from R. tomentosa inhibited proliferation on all cancer cell lines in a concentration-dependant manner. According to the IC50 obtained, the results exhibited significant activity against cancer cell line under 72h incubation with IC50 value < 30 μg/mL for ethyl acetate on HepG2, methanol and ethyl acetate on both MCF-7 and HT 29. Methanol extracts show significant antioxidant activities in DPPH, CUPRAC and β-carotene due to the presence of high total flavonoid and total phenolic content of R. tomentosa. Bioassay guided fractionation of ethyl acetate extract led to the isolation of lupeol and fractionation of chloroform extract led to the isolation of β-sitosterol. The structure of the isolated compounds were elucidated by spectroscopic methods such as 1D (1H, 13C, DEPTQ), 2D (COSY, HMQC, HMBC) NMR, MS, UV, FTIR and also by comparison with the literature. Collectively, the results show that R. tomentosa is a potential source of antioxidant and cytotoxicity and identified lupeol being a possible promising chemical lead for further development as anticancer agent.
610 2 0 _aFaculty of Industrial Sciences and Technology
_xDissertations
650 0 _aUniversities and Colleges
_xDissertations
650 0 _aTheses
999 _aVIRTUA40
_c7658
_d7664
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5000*5020*5040*5200*6100*6500*6501*9992